Immunogenicity and safety of virus-like particle of the porcine encephalomyocarditis virus in pig

Virology Journal

Dong-Jun An²  Hee Soo Lee²  Hwan-Won Choi³  Bo-Hye Shin²  WooSeog Jeong²  Won-Ha Lee¹  Hye-Young Jeoung¹ 

[1] Department of Genetic Engineering, School of Life Sciences and Biotechnology, Kyungpook National University, 1370 San-Kyuk-dong, Daegu 702-701, Republic of Korea;National Veterinary Research and Quarantine Service, Anyang, Gyeonggi-do, 430-824. Republic of Korea;Choongang Vaccine Laboratory, Daejeon, 305-348, Republic of Korea

关键词: vaccine candidate;    virus-like particles;    EMCV;   

Others  :  1156830
DOI  :  10.1186/1743-422X-8-170

 received in 2010-12-13, accepted in 2011-04-15,  发布年份 2011

【 摘 要 】

Background

In this study, porcine encephalomyocarditis virus (EMCV) virus-like particles (VLPs) were generated using a baculovirus expression system and were tested for immunogenicity and protective efficacy in vivo.

Results

VLPs were successfully generated from Sf9 cells infected with recombinant baculovirus and were confirmed to be approximately 30-40 nm by transmission electron microscopy (TEM). Immunization of mice with 0.5 μg crude protein containing the VLPs resulted in significant protection from EMCV infection (90%). In swine, increased neutralizing antibody titers were observed following twice immunization with 2.0 μg crude protein containing VLPs. In addition, high levels of neutralizing antibodies (from 64 to 512 fold) were maintained during a test period following the second immunization. No severe injection site reactions were observed after immunization and all swine were healthy during the immunization period

Conclusion

Recombinant EMCV VLPs could represent a new vaccine candidate to protect against EMCV infection in pig farms.

【 授权许可】

   
2011 Jeoung et al; licensee BioMed Central Ltd.

【 图 表 】

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