Viral metagenomic analysis of feces of wild small carnivores

Virology Journal

Saskia L Smits³  Albert DME Osterhaus³  Judith MA van den Brand¹  Claudia ME Schapendonk¹  Aritz Ruiz-Gonzalez²  Rogier Bodewes¹ 

[1] Department of Viroscience, Erasmus Medical Center, Rotterdam, The Netherlands;Conservation Genetics Laboratory, National Institute for Environmental Protection and Research (ISPRA), Bologna, Ozzano dell’Emilia (BO), Italy;ViroClinics BioSciences BV, Rotterdam, The Netherlands

关键词: Bunyavirus;    Common gennet;    Otter;    Fox;    Red fox;    Wildlife;    Wild carnivore;    Carnivore;    Phlebovirus;    Amdovirus;    Picobirnavirus;    Viral metagenomics;    Theilovirus;   

Others  :  804349
DOI  :  10.1186/1743-422X-11-89

 received in 2014-02-17, accepted in 2014-05-08,  发布年份 2014

【 摘 要 】

Background

Recent studies have clearly demonstrated the enormous virus diversity that exists among wild animals. This exemplifies the required expansion of our knowledge of the virus diversity present in wildlife, as well as the potential transmission of these viruses to domestic animals or humans.

Methods

In the present study we evaluated the viral diversity of fecal samples (n = 42) collected from 10 different species of wild small carnivores inhabiting the northern part of Spain using random PCR in combination with next-generation sequencing. Samples were collected from American mink (Neovison vison), European mink (Mustela lutreola), European polecat (Mustela putorius), European pine marten (Martes martes), stone marten (Martes foina), Eurasian otter (Lutra lutra) and Eurasian badger (Meles meles) of the family of Mustelidae; common genet (Genetta genetta) of the family of Viverridae; red fox (Vulpes vulpes) of the family of Canidae and European wild cat (Felis silvestris) of the family of Felidae.

Results

A number of sequences of possible novel viruses or virus variants were detected, including a theilovirus, phleboviruses, an amdovirus, a kobuvirus and picobirnaviruses.

Conclusions

Using random PCR in combination with next generation sequencing, sequences of various novel viruses or virus variants were detected in fecal samples collected from Spanish carnivores. Detected novel viruses highlight the viral diversity that is present in fecal material of wild carnivores.

【 授权许可】

   
2014 Bodewes et al.; licensee BioMed Central Ltd.

【 图 表 】

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