Insulin induces a positive relationship between the rates of ATP and glycogen changes in isolated rat liver in presence of glucose; a 31P and 13C NMR study

Nutrition & Metabolism

Jean-Louis Gallis²  Vincent Rigalleau²  Henri Gin²  Marie-Christine Beauvieux²  Laurence Baillet-Blanco¹ 

[1] Service de Diabétologie-Nutrition, Hôpital du Haut-Lévêque, Avenue de Magellan, F-33604 Pessac, France;Centre de Résonance Magnétique des Systèmes Biologiques, UMR 5536 CNRS-Université Bordeaux 2, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France

关键词: liver;    oxidative phosphorylation;    glycogen;    ATP;    insulin;   

Others  :  1138298
DOI  :  10.1186/1743-7075-2-32

 received in 2005-08-23, accepted in 2005-11-21,  发布年份 2005

【 摘 要 】

Background

There is an emerging theory suggesting that insulin, which is known to be the predominant postprandial anabolic hormone, is also a major regulator of mitochondrial oxidative phosphorylation in human skeletal muscle. However, little is known about its effects in the liver. Since there is a theoretical relationship between glycogen metabolism and energy status, a simultaneous and continuous investigation of hepatic ATP and glycogen content was performed in intact and isolated perfused liver by ³¹P and ¹³C nuclear magnetic resonance (NMR) The hepatic rates of ATP and glycogen changes were evaluated with different concentrations of insulin and glucose during continuous and short-term supply.

Results

Liver from rats fed ad libitum were perfused with Krebs-Henseleit Buffer (KHB)(controls) or KHB containing 6 mM glucose, 30 mM glucose, insulin alone, insulin + 6 mM glucose, insulin + 30 mM glucose. In the control, glycogenolysis occurred at a rate of -0.53 ± 0.021 %·min^-1 and ATP content decreased at a rate of -0.28 ± 0.029 %·min^-1. In the absence of insulin, there was a close proportional relationship between the glycogen flux and the glucose concentration, whereas ATP rates never varied. With insulin + glucose, both glycogen and ATP rates were strongly related to the glucose concentration; the magnitude of net glycogen flux was linearly correlated to the magnitude of net ATP flux: flux_glycogen = 72.543(flux_ATP) + 172.08, R² = 0.98.

Conclusion

Only the co-infusion of 30 mM glucose and insulin led to (i) a net glycogen synthesis, (ii) the maintenance of the hepatic ATP content, and a strong positive correlation between their net fluxes. This has never previously been reported. The specific effect of insulin on ATP change is likely related to a rapid stimulation of the hepatic mitochondrial oxidative phosphorylation. We propose that variations in the correlation between rates of ATP and glycogen changes could be a probe for insulin resistance due to the action of substrates, drugs or pathologic situations. Consequently, any work evaluating insulin resistance on isolated organs or in vivo should determine both ATP and glycogen fluxes.

【 授权许可】

   
2005 Baillet-Blanco et al; licensee BioMed Central Ltd.

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