Local production of CCL3, CCL11, and IFN-γ correlates with disease severity in murine parainfluenza virus infection

Virology Journal

Joseph B Domachowske²  Helene F Rosenberg¹  Cynthia A Bonville²  Manika Suryadevara² 

[1] Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA;Department of Pediatrics, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA

关键词: Interferon-γ;    Eotaxin;    CCL11;    MIP-1α;    CCL3;    Parainfluenza virus;    Sendai virus;   

Others  :  819981
DOI  :  10.1186/1743-422X-10-357

 received in 2013-06-25, accepted in 2013-12-10,  发布年份 2013

【 摘 要 】

Background

Using a murine model of parainfluenza virus infection (mPIV1 or Sendai virus; SeV), we compared the inflammatory responses to lethal and sub-lethal infections in inbred DBA/2 mice.

Methods

Mice were intranasally inoculated with either 1.6×10³ or 1.6×10⁵ infectious units (IU) of SeV or diluent control. Clinical data including daily weights, oxygen saturation, and lung function via whole body plethysmography were collected on days 0, 3–7, and 9–14. Clarified whole lung homogenates were evaluated for inflammatory markers by enzyme-linked immunoassay (ELISA). Data were analyzed using ANOVA or Student t-tests, as appropriate.

Results

Mice inoculated with 1.6×10⁵ IU of SeV developed a lethal infection with 100% mortality by day 7, while mice inoculated with 1.6×10³ IU developed a clinically significant infection, with universal weight loss but only 32% mortality. Interestingly, peak virus recovery from the lungs of mice inoculated with 1.6×10⁵ IU of SeV did not differ substantially from that detected in mice that received the 100-fold lower inoculum. In contrast, concentrations of CCL5 (RANTES), CCL11 (eotaxin), interferon-γ, CXCL10 (IP-10), and CCL3 (MIP-1α) were significantly higher in lung tissue homogenates from mice inoculated with 1.6×10⁵ IU (p < 0.05). In the lethal infection, levels of CCL11, interferon- γ and CCL3 all correlated strongly with disease severity.

Conclusion

We observed that severity of SeV-infection in DBA/2 mice was not associated with virus recovery but rather with the levels of proinflammatory cytokines, specifically CCL11, interferon- γ and CCL3, detected in lung tissue in response to SeV infection.

【 授权许可】

   
2013 Suryadevara et al.; licensee BioMed Central Ltd.

【 图 表 】

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