Full sequence analysis and characterization of the South Korean Norovirus GII-4 variant CUK-3

Virology Journal

Soon-Young Paik³  Sangryeol Ryu⁴  Weon-Hwa Jheong²  Young-Min Lee¹  Sung-Geun Lee³  Jeong-Woong Park⁴ 

[1] Department of Microbiology, College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Chungcheongbuk-do 361-763, Republic of Korea;Environmental Infrastructure Research Department, National Institute of Environmental Research, Incheon, 404-708, Republic of Korea;Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea;Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea

关键词: Phylogenetic tree;    Sequence;    Norovirus;   

Others  :  1156839
DOI  :  10.1186/1743-422X-8-167

 received in 2010-10-28, accepted in 2011-04-14,  发布年份 2011

【 摘 要 】

Background

Many of researchers have focused on the emerging pathogen, Norovirus, since its first identification as the causing agent of nonbacterial acute gastroenteritis in humans. One of the virulence factors of norovirus, the great genetic diversity attributed to point mutations and recombinations, has brought forth the result of significant changes in the circulating norovirus genotype patterns.

Findings

In recognition of the necessity for tracking and monitoring of genetic diversity, a norovirus variant among the most prevalent genotype GII-4, Norovirus Hu/GII-4/CUK-3/2008/KR (CUK-3), was isolated from stool samples and analyzed on the level of whole genome sequence. Whole genome sequence analysis revealed three ORF composites of the whole genome, ORF1 (5100 bp), ORF2 (1623 bp), and ORF3 (807 bp). Each genetic relationship of CUK-3 variant analysis located the ORF1 (5,100 bp) in Cluster I, ORF2 (1623 bp) in Cluster I (2006b), ORF3 (807 bp) in Cluster I, and the whole genome sequence (about 5.1 kb) in Cluster I in the phylogenetic tree. And the phylogenetic analyses showed the same location of CUK-3 strain with the GII-4/2006b cluster in the phylogenetic tree.

Conclusions

In This study, a first concerning the full-length sequence of a NoV variant in South Korea is meaningful in that it can be used not only as a full-length NoV variant sequence standard for future comparison studies, but also as useful material for the public health field by enabling the diagnosis, vaccine development, and prediction of new emerging variants.

【 授权许可】

   
2011 Park et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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