Experimental infection of Bama miniature pigs with a highly virulent classical swine fever virus

Virology Journal

Hua-Ji Qiu¹  Lian-Dong Qu¹  Zhuo Zhang¹  Shou-Ping Hu¹  Chang-De Si¹  Wen Han¹  Qiu-Ying Han¹  Hong Li¹  Huan Lin¹  Da-Yong Tian¹  Qian Jiang¹  Yuan Sun¹ 

[1] State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China

关键词: infection model;    classical swine fever virus;    Bama miniature pigs;   

Others  :  1156011
DOI  :  10.1186/1743-422X-8-452

 received in 2011-07-13, accepted in 2011-09-25,  发布年份 2011

【 摘 要 】

Background

Currently, larger domestic pigs are only animals widely used in vaccine evaluation and pathogenicity study of classical swine fever virus (CSFV). This study was aimed to create an alternative animal experimental infection model of CSFV.

Results

Twenty specific-pathogen-free Bama miniature pigs were randomly divided into two groups and rooms, infected and non-infected, and the pigs in the infected group were inoculated intramuscularly with 10⁴, 10⁵ or 10⁶ TCID₅₀ (median tissue culture infective dose) CSFV Shimen strain (n = 5 × 3) or left uninoculated to serve as in-contact pigs (n = 3). The uninfected control pigs (n = 2) were housed in a separate room. Clinical signs, body temperature, viraemia, tissue antigen distribution, pathological changes and seroconversion were monitored. Clinical signs were observed as early as 2 days post-inoculation (dpi) in all infected pigs (though mild in contact pigs), but not non-infected control pigs. All inoculated pigs showed viraemia by 6 dpi. The in-contact pigs showed lower levels of viraemia. At 10 dpi, seroconversion was noted in five of the 15 inoculated pigs. All inoculated or one in-contact pigs died by 15 dpi.

Conclusions

These results show that Bama miniature pigs support productive CSFV infection and display clinical signs and pathological changes consistent with CSFV infections observed in larger domestic pigs.

【 授权许可】

   
2011 Sun et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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