Anti-inflammatory activity of Chios mastic gum is associated with inhibition of TNF-alpha induced oxidative stress

Nutrition Journal

Sergey Dikalov²  Stamatios Lerakis²  Rafal Nazarewicz²  Anna Dikalova²  Alfiya Bikineyeva²  Angelike Triantafyllou¹ 

[1] Medical School of Athens, Athens, Greece;Division of Cardiology, Emory University School of Medicine, Atlanta, Georgia, USA

关键词: angiotensin II;    TNF-alpha;    NADPH oxidase;    protein kinase C;    hydrogen peroxide;    superoxide;    Chios mastic gum;    antioxidant;    oxidative stress;    Inflammation;   

Others  :  829602
DOI  :  10.1186/1475-2891-10-64

 received in 2010-12-23, accepted in 2011-06-06,  发布年份 2011

【 摘 要 】

Background

Gum of Chios mastic (Pistacia lentiscus var. chia) is a natural antimicrobial agent that has found extensive use in pharmaceutical products and as a nutritional supplement. The molecular mechanisms of its anti-inflammatory activity, however, are not clear. In this work, the potential role of antioxidant activity of Chios mastic gum has been evaluated.

Methods

Scavenging of superoxide radical was investigated by electron spin resonance and spin trapping technique using EMPO spin trap in xanthine oxidase system. Superoxide production in endothelial and smooth muscle cells stimulated with TNF-α or angiotensin II and treated with vehicle (DMSO) or mastic gum (0.1-10 μg/ml) was measured by DHE and HPLC. Cellular H₂O₂ was measured by Amplex Red. Inhibition of protein kinase C (PKC) with mastic gum was determined by the decrease of purified PKC activity, by inhibition of PKC activity in cellular homogenate and by attenuation of superoxide production in cells treated with PKC activator phorbol 12-myristate 13-acetate (PMA).

Results

Spin trapping study did not show significant scavenging of superoxide by mastic gum itself. However, mastic gum inhibited cellular production of superoxide and H₂O₂ in dose dependent manner in TNF-α treated rat aortic smooth muscle cells but did not affect unstimulated cells. TNF-α significantly increased the cellular superoxide production by NADPH oxidase, while mastic gum completely abolished this stimulation. Mastic gum inhibited the activity of purified PKC, decreased PKC activity in cell homogenate, and attenuated superoxide production in cells stimulated with PKC activator PMA and PKC-dependent angiotensin II in endothelial cells.

Conclusion

We suggest that mastic gum inhibits PKC which attenuates production of superoxide and H₂O₂ by NADPH oxidases. This antioxidant property may have direct implication to the anti-inflammatory activity of the Chios mastic gum.

【 授权许可】

   
2011 Triantafyllou et al; licensee BioMed Central Ltd.

【 图 表 】

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