Identification of canine parvovirus with the Q370R point mutation in the VP2 gene from a giant panda (Ailuropoda melanoleuca)

Virology Journal

Qi-gui Yan⁴  Manli Zhang²  Zifang Quan²  Zhongxiang Hao²  Wanzhu Guo²  Sanjie Cao²  Xintian wen²  Yupeng Ren⁵  Rong Hou³  Chengdong Wang³  Zhihe Zhang³  Shi-jie Chen¹  Shao-lin Yang²  Ling Guo² 

[1] Entry-exit Inspection and Quarantine Bureau, Chengdu, China;College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, China;Chengdu Research Base for Giant Panda Breeding, Chengdu, China;Key laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Ya’an, China;Southwest University for Nationalities, Chengdu, Sichuan, China

关键词: Giant panda;    Phylogenetic analysis;    Point mutation;    VP2 gene;    Canine parvovirus;   

Others  :  1150169
DOI  :  10.1186/1743-422X-10-163

 received in 2013-02-28, accepted in 2013-05-22,  发布年份 2013

【 摘 要 】

Background

In this study, we sequenced and phylogenetic analyses of the VP2 genes from twelve canine parvovirus (CPV) strains obtained from eleven domestic dogs and a giant panda (Ailuropoda melanoleuca) in China. A novel canine parvovirus (CPV) was detected from the giant panda in China.

Results

Nucleotide and phylogenetic analysis of the capsid protein VP2 gene classified the CPV as a new CPV-2a type. Substitution of Gln for Arg at the conserved 370 residue in CPV presents an unusual variation in the new CPV-2a amino acid sequence of the giant panda and is further evidence for the continuing evolution of the virus.

Conclusions

These findings extend the knowledge on CPV molecular epidemiology of particular relevance to wild carnivores.

【 授权许可】

   
2013 guo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Decaro N, Buonavoglia C: Canine parvovirus—A review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol 2012, 155:1-12.
• [2]Kang BK, Song DS, Lee CS, Jung KI, Park SJ, Kim EM, Park BK: Prevalence and genetic characterization of canine parvoviruses in Korea. Virus Genes 2008, 36:127-133.
• [3]Decaro N, Elia G, Campolo M, Desario C, Lucente M, Bellacicco A, Buonavoglia C: New approaches for the molecular characterization of canine parvovirus type 2 strains. J Vet Med B 2005, 52:316-319.
• [4]Parrish CR, Aquadro CF, Strassheim M, Evermann J, Sgro J, Mohammed H: Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol 1991, 65:6544-6552.
• [5]Parrish CR, O’Connell PH, Evermann JF, Carmichael LE: Natural variation of canine parvovirus. Science 1985, 230:1046-1048.
• [6]Parker JSL, Murphy WJ, Wang D, O’Brien SJ, Parrish CR: Canine and feline parvoviruses can use human or feline transferrin receptors to bind, enter, and infect cells. J Virol 2001, 75:3896-3902.
• [7]Truyen U: Evolution of canine parvovirus: loss and gain of the feline host]. Tierarztl Prax 1996, 24:316.
• [8]Martella V, Cavalli A, Decaro N, Elia G, Desario C, Campolo M, Bozzo G, Tarsitano E, Buonavoglia C: Immunogenicity of an intranasally administered modified live canine parvovirus type 2b vaccine in pups with maternally derived antibodies. Clin Diagn Lab Immun 2005, 12:1243-1245.
• [9]Ohshima T, Hisaka M, Kawakami K, Kishi M, Tohya Y, Mochizuki M: Chronological analysis of canine parvovirus type 2 isolates in Japan. J Vet Med Sci Japan Soc Vet Sci 2008, 70:769.
• [10]Truyen U: Evolution of canine parvovirus–a need for new vaccines? Vet Microbiol 2006, 117:9-13.
• [11]Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D, Bozzo G, Elia G, Decaro N, Carmichael L: Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol 2001, 82:3021.
• [12]Decaro N, Martella V, Desario C, Bellacicco A, Camero M, Manna L, d’Aloja D, Buonavoglia C: First detection of canine parvovirus type 2c in pups with haemorrhagic enteritis in Spain. J Vet Med B 2006, 53:468-472.
• [13]Nakamura M, Tohya Y, Miyazawa T, Mochizuki M, Phung H, Nguyen N, Huynh L, Nguyen L, Nguyen P, Nguyen P: A novel antigenic variant of canine parvovirus from a Vietnamese dog. Arch Virol 2004, 149:2261-2269.
• [14]Touihri L, Bouzid I, Daoud R, Desario C, El Goulli AF, Decaro N, Ghorbel A, Buonavoglia C, Bahloul C: Molecular characterization of canine parvovirus-2 variants circulating in Tunisia. Virus Genes 2009, 38:249-258.
• [15]Decaro N, Desario C, Addie DD, Martella V, Vieira MJ, Elia G, Zicola A, Davis C, Thompson G, Thiry E: Molecular epidemiology of canine parvovirus, Europe. Emerg Infect Dis 2007, 13:1222-1224.
• [16]Decaro N, Desario C, Amorisco F, Losurdo M, Elia G, Parisi A, Ventrella G, Martella V, Buonavoglia C: Detection of a canine parvovirus type 2c with a non-coding mutation and its implications for molecular characterisation. Vet J 2013. (In Press)
• [17]Hong C, Decaro N, Desario C, Tanner P, Pardo MC, Sanchez S, Buonavoglia C, Saliki JT: Occurrence of canine parvovirus type 2c in the United States. J Vet Diagen Invest 2007, 19:535-539.
• [18]Wu J, Cao GW, Jiang YK, Luo JM, Yang SQ, Wu GQ, Huang XW, He GX, Li YS, Ye ZY: Serosurvey of infection of parvovirus in giant pandas. Chin J Preventive Vet Med 1988, 2:38-39.
• [19]Mainka SA, Qiu X, He T, Appel MJ: Serologic survey of giant pandas (Ailuropoda melanoleuca), and domestic dogs and cats in the Wolong Reserve, China. J Wildlife Dis 1994, 30:86-89.
• [20]Loeffler IK, Howard J, Montali RJ, Hayek L-A, Dubovi E, Zhang Z, Yan Q, Guo W, Wildt DE: Serosurvey of ex situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications for species conservation. J Zoo Wildlife Med 2007, 38:559-566.
• [21]Qin Q, Li D, Zhang H, Hou R, Zhang Z, Zhang C, Zhang J, Wei F: Serosurvey of selected viruses in captive giant pandas (Ailuropoda melanoleuca) in China. Vet Microbiol 2010, 142:199-204.
• [22]Chinchkar S, Mohana Subramanian B, Hanumantha Rao N, Rangarajan P, Thiagarajan D, Srinivasan V: Analysis of VP2 gene sequences of canine parvovirus isolates in India. Arch Virol 2006, 151:1881-1887.
• [23]Clegg S, Coyne K, Parker J, Dawson S, Godsall S, Pinchbeck G, Cripps P, Gaskell R, Radford A: Molecular Epidemiology and Phylogeny Reveal Complex Spatial Dynamics in Areas Where Canine Parvovirus Is Endemic. J Virol 2011, 85:7892-7899.
• [24]Martella V, Decaro N, Elia G, Buonavoglia C: Surveillance activity for canine parvovirus in Italy. J Vet Med B 2005, 52:312-315.
• [25]Mochizuki M, Ohshima T, Une Y, Yachi A: Recombination between vaccine and field strains of Canine Parvovirus is revealed by isolation of virus in canine and feline cell cultures. J Vet Med Sci 2008, 70:1305-1314.
• [26]Decaro N, Desario C, Billi M, Mari V, Elia G, Cavalli A, Martella V, Buonavoglia C: Western European epidemiological survey for parvovirus and coronavirus infections in dogs. Vet J 2011, 187:195-199.
• [27]Zhang R, Yang S, Zhang W, Zhang T, Xie Z, Feng H, Wang S, Xia X: Phylogenetic analysis of the VP2 gene of canine parvoviruses circulating in China. Virus Genes 2010, 40:397-402.
• [28]Ikeda Y, Mochizuki M, Naito R, Nakamura K, Miyazawa T, Mikami T, Takahashi E: Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats. Virology 2000, 278:13-19.
• [29]Battilani M, Scagliarini A, Tisato E, Turilli C, Jacoboni I, Casadio R, Prosperi S: Analysis of canine parvovirus sequences from wolves and dogs isolated in Italy. J Gen Virol 2001, 82:1555-1560.
• [30]Jeoung S-Y, Ahn S-J, Kim D: Genetic analysis of VP2 gene of canine parvovirus isolates in Korea. J Vet Med Sci Japan Soc Vet Sci 2008, 70:719.
• [31]Decaro N, Desario C, Parisi A, Martella V, Lorusso A, Miccolupo A, Mari V, Loredana Colaianni M, Cavalli A, Di Trani L: Genetic analysis of canine parvovirus type 2c. Virology 2009, 385:5-10.
• [32]Hoelzer K, Shackelton LA, Parrish CR, Holmes EC: Phylogenetic analysis reveals the emergence, evolution and dispersal of carnivore parvoviruses. J Gen Virol 2008, 89:2280-2289.
• [33]Stucker KM, Pagan I, Cifuente JO, Kaelber JT, Lillie TD, Hafenstein S, Holmes EC, Parrish CR: The role of evolutionary intermediates in the host adaptation of canine parvovirus. J Virol 2012, 86:1514-1521.
• [34]Tsao J, Chapman MS, Agbandje M, Keller W, Smith K, Wu H, Luo M, Smith TJ, Rossmann MG, Compans RW: The three-dimensional structure of canine parvovirus and its functional implications. Science 1991, 251:1456-1464.
• [35]Kaelber JT, Demogines A, Harbison CE, Allison AB, Goodman LB, Ortega AN, Sawyer SL, Parrish CR: Evolutionary reconstructions of the transferrin receptor of Caniforms supports canine parvovirus being a re-emerged and not a novel pathogen in dogs. PLoS Pathog 2012, 8:e1002666.
• [36]Pereira CAD, Leal ES, Durigon EL: Selective regimen shift and demographic growth increase associated with the emergence of high-fitness variants of canine parvovirus. Infect Genet Evol 2007, 7:399-409.
• [37]Elia G, Cavalli A, Cirone F, Lorusso E, Camero M, Buonavoglia D, Tempesta M: Antibody levels and protection to canine parvovirus type 2. J Vet Med B 2005, 52:320-322.
• [38]Decaro N, Cirone F, Desario C, Elia G, Lorusso E, Colaianni M, Martella V, Buonavoglia C: Severe parvovirus in a 12-year-old dog that had been repeatedly vaccinated. Vet Rec 2009, 164:593-595.
• [39]Decaro N, Desario C, Elia G, Martella V, Mari V, Lavazza A, Nardi M, Buonavoglia C: Evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c. Microbiol-Quart J Microbiol Sci 2008, 31:125-130.
• [40]Cavalli A, Martella V, Desario C, Camero M, Bellacicco AL, De Palo P, Decaro N, Elia G, Buonavoglia C: Evaluation of the antigenic relationships among canine parvovirus type 2 variants. Clin Vaccine Immunol 2008, 15:534-539.
• [41]Wang CD, Zhang ZH: Current status of canine distemper vaccine immune among giant pandas and red pandas. Sichuan J Zool 2006, 25:668-672.
• [42]Decaro N, Desario C, Campolo M, Elia G, Martella V, Ricci D, Lorusso E, Buonavoglia C: Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant. J Vet Diagen Invest 2005, 17:133.
• [43]Schunck B, Kraft W, Truyen U: A simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukopenia virus in feces. J Virol Met 1995, 55:427-433.
• [44]Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011, 28:2731-2739.