Rickettsia bellii, Rickettsia amblyommii, and Laguna Negra hantavirus in an Indian reserve in the Brazilian Amazon

Particle and Fibre Toxicology

Elba Regina Sampaio de Lemos¹  Jairo Dias Barreira⁶  Paulo Sérgio D’Andrea⁵  Cibele Rodrigues Bonvicino⁴  Alba Valéria Gomes de Melo Via²  Valéria Cristina da Silva²  Ana Beatriz M V Almeida³  Sebastião de Jesus³  Inês Anschau³  José Ferreira Figueredo³  Jorlan Fernandes¹  Maria Angélica Mares-Guia¹  Renata Carvalho de Oliveira¹  Tatiana Rozental¹  Alexandro Guterres¹  Lívia de Barros Lopes¹ 

[1] Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ 21045-900, Brazil;Secretaria Estadual de Saúde, SES, Mato Grosso, Brazil;Fundação Nacional de Saúde, FUNASA-DSEI, Mato Grosso, Brazil;Programa de Genética, Instituto Nacional de Câncer, Ministério da Saúde, Rio de Janeiro, Brazil;Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil;Universidade Federal do Estado do Rio de Janeiro, UNIRIO, Rio de Janeiro, Brazil

关键词: Laguna negra virus;    Rickettsia amblyommii;    Rickettsia bellii;    Indian population;   

Others  :  807161
DOI  :  10.1186/1756-3305-7-191

 received in 2014-01-24, accepted in 2014-04-08,  发布年份 2014

【 摘 要 】

Background

The purpose of this study was to identify the presence of rickettsia and hantavirus in wild rodents and arthropods in response to an outbreak of acute unidentified febrile illness among Indians in the Halataikwa Indian Reserve, northwest of the Mato Grosso state, in the Brazilian Amazon. Where previously surveillance data showed serologic evidence of rickettsia and hantavirus human infection.

Methods

The arthropods were collected from the healthy Indian population and by flagging vegetation in grassland or woodland along the peridomestic environment of the Indian reserve. Wild rodents were live-trapped in an area bordering the reserve limits, due the impossibility of capturing wild animals in the Indian reserve. The wild rodents were identified based on external and cranial morphology and karyotype. DNA was extracted from spleen or liver samples of rodents and from invertebrate (tick and louse) pools, and the molecular characterization of the rickettsia was through PCR and DNA sequencing of fragments of two rickettsial genes (gltA and ompA). In relation to hantavirus, rodent serum samples were serologically screened by IgG ELISA using the Araraquara-N antigen and total RNA was extracted from lung samples of IgG-positive rodents. The amplification of the complete S segment was performed.

Results

A total of 153 wild rodents, 121 louse, and 36 tick specimens were collected in 2010. Laguna Negra hantavirus was identified in Calomys callidus rodents and Rickettsia bellii, Rickettsia amblyommii were identified in Amblyomma cajennense ticks.

Conclusions

Zoonotic diseases such as HCPS and spotted fever rickettsiosis are a public health threat and should be considered in outbreaks and acute febrile illnesses among Indian populations. The presence of the genome of rickettsias and hantavirus in animals in this Indian reserve reinforces the need to include these infectious agents in outbreak investigations of febrile cases in Indian populations.

【 授权许可】

   
2014 de Barros Lopes et al.; licensee BioMed Central Ltd.

【 图 表 】

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