DNA barcoding does not separate South American Triatoma (Hemiptera: Reduviidae), Chagas Disease vectors

Particle and Fibre Toxicology

Cleber Galvão¹  Carolina Dale¹  Silvia Andrade Justi¹ 

[1] Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos, InstitutoOswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil

关键词: Molecular identification;    DNA barcoding;    Chagas disease;    Triatominae;   

Others  :  1149113
DOI  :  10.1186/s13071-014-0519-1

 received in 2014-09-13, accepted in 2014-11-04,  发布年份 2014

【 摘 要 】

Background

DNA barcoding assumes that a biological entity is completely separated from its closest relatives by a barcoding gap, which means that intraspecific genetic distance (from COI sequences) should never be greater than interspecific distances. We investigated the applicability of this strategy in identifying species of the genus Triatoma from South America.

Findings

We calculated intra and interspecific Kimura-2-parameter distances between species from the infestans, matogrossensis, sordida and rubrovaria subcomplexes. In every subcomplex examined we observed at least one intraspecific distance greater than interspecific distances.

Conclusions

Although DNA barcoding is a straightforward approach, it was not applicable for identifying Southern American Triatoma species, which may have diverged recently. Thus, caution should be taken in identifying vector species using this approach, especially in groups where accurate identification of taxa is fundamentally linked to public health issues.

【 授权许可】

   
2014 Justi et al.; licensee BioMed Central.

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