Helminth control in kennels: is the combination of milbemycin oxime and praziquantel a right choice?

Particle and Fibre Toxicology

Giuseppe Cringoli³  Francesco La Torre¹  Maria Paola Maurelli³  Vincenzo Musella²  Saverio Pennacchio³  Laura Rinaldi³ 

[1] Faculty of Veterinary Medicine, University of Teramo, Piazza A. Moro, 45, Teramo, 64100, Italy;Department of Health Sciences, University Magna Græcia, Catanzaro, Italy;Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR, Naples, Regione Campania, Italy

关键词: Nemex® POP;    Drontal Plus Flavour®;    Milbemax®;    Control;    Cardio-pulmonary helminths;    Intestinal helminths;    Kennels;   

Others  :  1147786
DOI  :  10.1186/s13071-015-0647-2

 received in 2014-06-09, accepted in 2015-01-07,  发布年份 2015

【 摘 要 】

Background

Kennel dogs are at the high risk of infections with intestinal and extra-intestinal helminths. Therefore, regular parasitological surveillance, appropriate treatment strategies and high quality standard of hygiene are required to guarantee the health and welfare of kennel dogs. The aim of the present study was to evaluate the efficacy of helminth control in kennels using different broad-spectrum anthelmintics that are routinely used in canine veterinary practice. Particular attention was given to the field efficacy and ease-of-use of each product.

Methods

The study was conducted in 3 public kennels in the Campania region (southern Italy). Eighteen boxes from each of the three kennels were selected for treatment based on faecal egg counts (FECs) at Day −30. The treatments were conducted using tablets containing combinations of: i) milbemycin oxime and praziquantel (Milbemax®); ii) pyrantel embonate, febantel and praziquantel (Drontal Plus Flavour®), and; iii) pyrantel pamoate, oxantel pamoate and praziquantel (Nemex® POP). All dogs were treated on Day 0 and sampled on Days 0, 7, 14 and 21 for copromicroscopic analyses. The FLOTAC dual technique on pooled samples was used with efficacy determined by reduction in FECs.

Results

At Day −30 intestinal nematodes (hookworms, Toxocara, Trichuris) and cestodes (Dypilidium caninum) were found. Milbemax® showed 100% efficacy against all the helminths in all the kennels. Drontal Plus Flavour® was 100% effective against hookworms in all the kennels but gave lower efficacy against T. canis (range = 97.1-100%) and T. vulpis (range = 95.6-100%). Nemex® POP was also 100% effective against hookworms in all kennels but less effective against T. canis (range = 95.7-100%) and T. vulpis (range = 95.7-100%). All three drugs were 100% effective against D.caninum.

Conclusions

Milbemax®was fully effective against all the helminthes and was palatable and ease-of-use for all the dogs. It is the optimum choice for the treatment and prevention of helminth infections in kennel dogs under field conditions.

【 授权许可】

   
2015 Rinaldi et al.; licensee BioMed Central.

【 参考文献 】

• [1]Traversa D. Pet roundworms and hookworms: a continuing need for global worming. Parasit Vectors. 2012; 5:91. BioMed Central Full Text
• [2]Grandemange E, Claerebout E, Genchi C, Franc M. Field evaluation of the efficacy and the safety of a combination of oxantel/pyrantel/praziquantel in the treatment of naturally acquired gastrointestinal nematode and/or cestode infestations in dogs in Europe. Vet Parasitol. 2007; 145:94-9.
• [3]Traversa D. Are we paying too much attention to cardio-pulmonary nematodes and neglecting old-fashioned worms like Trichuris vulpis? Parasit Vectors. 2011; 4:32. BioMed Central Full Text
• [4]Traversa D, Frangipane di Regalbono A, Di Cesare A, La Torre F, Drake J, Pietrobelli M. Environmental contamination by canine geohelminths. Parasit Vectors. 2014; 7(1):67. BioMed Central Full Text
• [5]Morgan ER, Cavill L, Curry GE, Wood RM, Mitchell ES. Effects of aggregation and sample size on composite faecal egg counts in sheep. Vet Parasitol. 2005; 131:79-87.
• [6]Rinaldi L, Pennacchio S, Musella V, Maurelli MP, Guariglia I, Cappelli G et al.. Kennel dogs and helminth infections in the Campania region. In: Proceedings of the Italian Conference of the Italian Society of Parasitology (SOIPA): 26–29 June 2012. Rolando, editor. Alghero (SS), Italy; 2012: p.45.
• [7]Baldelli R, Piva S, Salvatore D, Parigi M, Melloni O, Tamba M et al.. Canine leishmaniasis surveillance in a northern Italy kennel. Vet Parasitol. 2011; 179:57-61.
• [8]Good clinical practice. Veterinary International Cooperation on Harmonization, European Agency for the Evaluation of Medicinal Products, London; 2000.
• [9]Efficacy requirements for anthelmintics: general requirements. Veterinary International Cooperation on Harmonization, European Agency for the Evaluation of Medicinal Products, London; 2000.
• [10]Efficacy of anthelmintics: specific recommendations for canine. Veterinary International Cooperation on Harmonization, European Agency for the Evaluation of Medicinal Products, London; 2001.
• [11]Jacobs DE, Arakawa A, Courtney CH, Gemmell MA, McCall JW, Myers GH et al.. World Association for the Advancement of Veterinary Parasitology (WAAVP) guidelines for evaluating the efficacy of anthelmintics in dogs and cats. Vet Parasitol. 1994; 52:179-202.
• [12]Cringoli G, Rinaldi L, Maurelli MP, Utzinger J. FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans. Nat Protoc. 2010; 5:503-15.
• [13]Cringoli G, Rinaldi L, Maurelli MP, Morgoglione ME, Musella V, Utzinger J. Ancylostoma caninum: calibration and comparison of diagnostic accuracy of flotation in tube, McMaster and FLOTAC in faecal samples of dogs. Exp Parasitol. 2011; 128(1):32-7.
• [14]Rinaldi L, Maurelli MP, Musella V, Santaniello A, Coles GC, Cringoli G et al.. An improved method for diagnosis of lungworm infections in sheep. Vet Parasitol. 2010; 169:395-8.
• [15]Schnyder M, Maurelli MP, Morgoglione ME, Kohler L, Deplazes P, Torgerson P, Cringoli G, Rinaldi L: Comparison of faecal techniques including FLOTAC for copromicroscopic detection of first stage larvae of Angiostrongylus vasorum. Parasitol Res. 109(1):63–69
• [16]Neves D, Lobo L, Simões PB, Cardoso L. Frequency of intestinal parasites in pet dogs from an urban area (Greater Oporto, northern Portugal). Vet Parasitol. 2014; 200(3–4):295-8.
• [17]Morgan E, Shaw S. Angiostrongylus vasorum infection in dogs: continuing spread and developments in diagnosis and treatment. J Small Anim Pract. 2010; 51(12):616-21.
• [18]Rinaldi L, Cringoli G. Exploring the interface between diagnostics and maps of neglected parasitic diseases. Parasitology. 2014; 28 Suppl 1:1-8.
• [19]Altreuther G, Radeloff I, LeSueur C, Schimmel A, Krieger KJ. Field evaluation of the efficacy and safety of emodepside plus praziquantel tablets (Profender tablets for dogs) against naturally acquired nematode and cestode infections in dogs. Parasitol Res. 2009; 105 Suppl 1:23-9.
• [20]Hellmann K, Knoppe T, Radeloff I, Heine J. The anthelmintic efficacy and the safety of a combination of imidacloprid and moxidectin spot-on in cats and dogs under field conditions in Europe. Parasitol Res. 2003; 90 Suppl 3:142-3.
• [21]Conboy G. Natural infections of Crenosoma vulpis and Angiostrongylus vasorum in dogs in Atlantic Canada and their treatment with milbemycinoxime. Vet Rec. 2004; 155:16-8.
• [22]Conboy G, Bourque A, Miller L, Seewald W, Schenker R. Efficacy of Milbemax (milbemycinoxime + praziquantel) in the treatment of dogs experimentally infected with Crenosoma vulpis. Vet Parasitol. 2013; 198(3–4):319-24.
• [23]Rinaldi L, Calabria G, Carbone S, Carrella A, Cringoli G. Crenosoma vulpis in dog: first case report in Italy and use of the FLOTAC technique for copromicroscopic diagnosis. Parasitol Res. 2007; 101:1681-4.
• [24]Motta B, Schnyder M, Basano FS, Nägeli F, Nägeli C, Schiessl B et al.. Therapeutic efficacy of milbemycinoxime/praziquantel oral formulation (Milbemax®) against Thelazia callipaeda in naturally infested dogs and cats. Parasit Vectors. 2012; 5:85. BioMed Central Full Text
• [25]Sager H, Moret CS, Grimm F, Deplazes P, Doherr MG, Gottstein B. Coprological study on intestinal helminths in Swiss dogs: temporal aspects of anthelminthic treatment. Parasitol Res. 2006; 98(4):333-8.
• [26]Balassiano BC, Campos MR, MenezesRde C, Pereira MJ. Factors associated with gastrointestinal parasite infection in dogs in Rio de Janeiro, Brazil. Prev Vet Med. 2009; 91(2–4):234-40.