Phlebotomus papatasi SP15: mRNA expression variability and amino acid sequence polymorphisms of field populations

Particle and Fibre Toxicology

Mary Ann McDowell⁴  Frank H. Collins⁴  Shaden Kamhawi¹  Scott J. Emrich⁴  Andrew R. Mahon³  Neil F. Lobo⁴  Jenica Abrudan⁷  Douglas A. Shoue⁴  Mariha Wadsworth⁴  Gwen Stayback⁴  David F. Hoel⁶  Mahmoud Abo-Shehada⁵  Emad El-Din Y. Fawaz⁹  Shabaan S. El-Hossary⁹  Hanafi A. Hanafi⁵  Hussan Dayem⁵  Rami Mukbel⁵  Carlos Alberto S. Figueiredo⁸  Valdir Q. Balbino⁸  Iliano V. Coutinho-Abreu¹  Marcelo Ramalho-Ortigão² 

[1] Laboratory of Malaria and Vector Research, NIAID-NIH, 12735 Twinbrook Parkway, Rockville 20852, MD, USA;Department of Entomology, Kansas State University, Manhattan 66506, KS, USA;Department of Biology, Central Michigan University, Mount Pleasant, Detroit 48859, MI, USA;Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame 46556, IN, USA;Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan;Department of Biostatistics, Uniformed Services University of the Health Sciences, Bethesda 20814, MD, USA;Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, USA;Department of Genetics, Universidade Federal de Pernambuco, Recife, PE, Brazil;Vector Biology Research Program, U.S. Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

关键词: MHC class II epitopes;    Expression variability;    Vaccine;    Leishmaniasis;    PpSP15;    Saliva;    Sand fly;   

Others  :  1224686
DOI  :  10.1186/s13071-015-0914-2

 received in 2015-04-11, accepted in 2015-05-22,  发布年份 2015

【 摘 要 】

Background

The Phlebotomus papatasi salivary protein PpSP15 was shown to protect mice against Leishmania major, suggesting that incorporation of salivary molecules in multi-component vaccines may be a viable strategy for anti-Leishmania vaccines.

Methods

Here, we investigated PpSP15 predicted amino acid sequence variability and mRNA profile of P. papatasi field populations from the Middle East. In addition, predicted MHC class II T-cell epitopes were obtained and compared to areas of amino acid sequence variability within the secreted protein.

Results

The analysis of PpSP15 expression from field populations revealed significant intra- and interpopulation variation.. In spite of the variability detected for P. papatasi populations, common epitopes for MHC class II binding are still present and may potentially be used to boost the response against Le. major infections.

Conclusions

Conserved epitopes of PpSP15 could potentially be used in the development of a salivary gland antigen-based vaccine.

【 授权许可】

   
2015 Ramalho-Ortigão et al.

【 图 表 】

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