High prevalence of Trichinella pseudospiralis in Florida panthers (Puma concolor coryi)

Particle and Fibre Toxicology

Edoardo Pozio⁵  Gianluca Marucci⁵  Maria Interisano⁵  Kenneth Logan⁴  Dave Onorato¹  Mark Cunningham¹  Jacqueline M Paritte²  Jennifer E Thomas²  Marc Criffield³  Mason V Reichard² 

[1] Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, 1105 SW Williston Rd, Gainesville 32601, FL, USA;Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, 250 McElroy Hall, Stillwater 74078, OK, USA;Fish and Wildlife Research Institute, Florida Fish and Wildlife Conservation Commission, 298 Sabal Palm Rd, Naples 34114, FL, USA;Colorado Parks and Wildlife, 2300 S. Townsend Ave, Montrose 81401, CO, USA;Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, Rome 00161, Italy

关键词: Zoonotic;    Trichinella pseudospiralis;    Trichinella spiralis;    Puma concolor coryi;    Florida panther;   

Others  :  1147206
DOI  :  10.1186/s13071-015-0674-z

 received in 2014-12-08, accepted in 2015-01-20,  发布年份 2015

【 摘 要 】

Background

Parasites of the genus Trichinella are zoonotic nematodes common in carnivores throughout the world. We determined the prevalence and species of Trichinella infections in Florida panthers (Puma concolor coryi).

Methods

Tongues from Florida panthers were collected at necropsy and examined by pepsin-HCl artificial digestion for infection with Trichinella spp. DNA was extracted from larvae and multiplex PCR using Trichinella species-specific primers was used to genotype the worms.

Results

Trichinella spp. larvae were detected in 24 of 112 (21.4%; 14.6%–30.3%) panthers. Sixteen of the panthers (14.3%) were infected with T. pseudospiralis, 1 (0.9%) was infected with T. spiralis, and 2 (1.8%) had mixed infections of T. pseudospiralis and T. spiralis. Trichinella spp. larvae from 5 panthers were not identified at the species level due to degraded DNA.

Conclusions

This is the highest prevalence of T. pseudospiralis detected in North America up to now and suggests the Florida panther is a key mammalian reservoir of this parasite in southern Florida. Trichinella pseudospiralis can infect both mammals and birds indicating the source of infection for Florida panthers could be broader than believed; however, birds represent a small percentage (0.01%) of the cat’s diet. Since wild pigs (Sus scrofa) can be parasitized by both T. pseudospiralis and T. spiralis and these swine can comprise a large portion (~40%) of a panther’s diet in Florida, we believe that Florida panthers acquired these zoonotic parasites from feeding on wild pigs.

【 授权许可】

   
2015 Reichard et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

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