Differential lumbar spinal cord responses among wild type, CD4 knockout, and CD40 knockout mice in spinal nerve L5 transection-induced neuropathic pain

Molecular Pain

Adriana Eurich¹  Holly Beaulac¹  Ling Cao¹ 

[1] Department of Biomedical Sciences, College of Osteopathic Medicine, University of New England, Biddeford, ME, USA

关键词: Microglia;    CD40;    CD4⁺ T cells;    Nerve injury;    Neuopathic pain;   

Others  :  862672
DOI  :  10.1186/1744-8069-8-88

 received in 2012-10-16, accepted in 2012-12-14,  发布年份 2012

【 摘 要 】

Background

Our previous studies have indicated that both lumbar spinal cord-infiltrating CD4⁺ T cells and microglial CD40 contribute to the maintenance of mechanical hypersensitivity in a murine model of neuropathic pain spinal nerve L5 transection (L5Tx). To further delineate the CD4 and CD40-mediated mechanisms involved in the development of L5Tx-induced neuropathic pain behaviors, we examined the lumbar spinal cord mononuclear cells of wild type (WT) BALB/c, BALB/c-CD4 knockout (KO), and BALB/c-CD40 KO mice via flow cytometry.

Results

In WT mice, L5Tx induced significant but transient (at day 3 and/or day 7) increases of the total numbers of mononuclear cells, microglial cells (CD45^loCD11b⁺), and infiltrating leukocytes (CD45^hi) in the ipsilateral side of the spinal cord. In CD4 KO mice, significant elevation of microglia was detected only on day 7 post-L5Tx, while no significant increase in infiltrating leukocytes post-L5Tx was observed. CD40 KO mice did not exhibit any of the changes observed in WT mice. Furthermore, neutralizing CD40 antibody treatment indicated an early involvement of CD40 signaling in the development of L5Tx-induced mechanical hypersensitivity.

Conclusions

Altogether, data indicate that both CD4 and CD40 play a role in L5Tx-induced leukocyte infiltration into the lumbar spinal cord but have differential contributions to spinal cord microglial activation following peripheral nerve injury.

【 授权许可】

   
2012 Cao et al.; licensee BioMed Central Ltd.

【 图 表 】

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