Chloroquine is a potent inhibitor of SARS coronavirus infection and spread

Virology Journal

Stuart T Nichol²  Nabil G Seidah¹  Thomas G Ksiazek²  Pierre E Rollin²  Bobbie R Erickson²  Suzanne Benjannet¹  Eric Bergeron¹  Martin J Vincent² 

[1] Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Ave West, Montreal, QCH2W1R7, Canada;Special Pathogens Brach, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia, 30333, USA

关键词: therapy;    inhibition;    chloroquine;    severe acute respiratory syndrome coronavirus;   

Others  :  1158150
DOI  :  10.1186/1743-422X-2-69

 received in 2005-07-12, accepted in 2005-08-22,  发布年份 2005

【 摘 要 】

Background

Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available.

Results

We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. In addition to the well-known functions of chloroquine such as elevations of endosomal pH, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations.

Conclusion

Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds.

【 授权许可】

   
2005 Vincent et al; licensee BioMed Central Ltd.

【 图 表 】

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