A transgenic ginseng vaccine for bovine viral diarrhea

Virology Journal

Lianxue Zhang¹  Ran Li¹  He Yang¹  Pu Zang¹  Chao Sun²  Xueliang Zhao¹  Yugang Gao¹ 

[1] College of Traditional Chinese Medicine, Jilin Agricultural University, ChangChun 130118, China;College of AnimCal Science and Technology, Northwest A & F University, Yang Ling 712100, China

关键词: Erns;    Transgenic ginseng vaccine;    Bovine viral diarrhea virus;   

Others  :  1224940
DOI  :  10.1186/s12985-015-0301-9

 received in 2014-08-31, accepted in 2015-04-21,  发布年份 2015

【 摘 要 】

Background

Bovine viral diarrhea virus (BVDV) infections are endemic in cattle populations worldwide and cause major economic losses. Thus, an effective vaccine is needed against the transmission of BVDV. The glycoprotein E^rns is one of the envelope proteins of this virus and shows BVDV-related immunogenicity. Here, we report the use of Panax ginseng as an alternative production platform for the expression of glycoprotein E^rns via Agrobacterium-mediated transformation.

Result

Polymerase chain reaction (PCR) and reverse transcription (RT)-PCR analyses showed that pBI121-E^rns was stably integrated into the chromosome of transformants. ELISA assay and Western blot analysis confirmed the antigenicity of plant-derived E^rns glycoprotein. Immunogenicity was evaluated subcutaneously in deer using a soluble protein extract of dried transgenic ginseng hairy roots. Specific humoral and cell-mediated immune responses against BVDV were detected following immunization.

Conclusion

These results demonstrated that the E^rns glycoprotein could be expressed in ginseng hairy roots and that plant-derived glycoprotein E^rns retained its antigenicity and immunogenicity.

【 授权许可】

   
2015 Gao et al.; licensee BioMed Central.

【 图 表 】

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