A comparison of high-throughput techniques for assaying circadian rhythms in plants

Plant Methods

Anthony Hall¹  James Hartwell¹  Peter D Gould¹  Mark Greenwood¹  Jade Waller¹  Andrew J Tindall¹ 

[1] Institute of Integrative Biology, University of Liverpool, Crown Street, Liverpool, UK

关键词: High-throughput assay;    Circadian clock;    Infra-red gas exchange;    Delayed fluorescence;    Leaf movement;    Luciferase;   

Others  :  1204410
DOI  :  10.1186/s13007-015-0071-9

 received in 2015-03-05, accepted in 2015-04-02,  发布年份 2015

【 摘 要 】

Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With the circadian clock increasingly implicated in key fitness and signalling pathways, this has opened up new avenues for understanding plant development and signalling. Our tool-kit has been constantly improving through continual development and novel techniques that increase throughput, reduce costs and allow higher resolution on the cellular and subcellular levels. With circadian assays becoming more accessible and relevant than ever to researchers, in this paper we offer a review of the techniques currently available before considering the horizons in circadian investigation at ever higher throughputs and resolutions.

【 授权许可】

   
2015 Tindall et al.; licensee BioMed Central.

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