Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.)

Proteome Science

Elaheh Vatankhah³  Javad Gharechahi²  Behzad Ghareyazie¹  Hassan Ebrahimzadeh³  Golandam Sharifi³ 

[1] Department of Genomics, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran;Department of Molecular Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran;Department of Botany, Faculty of Science, University of Tehran, Tehran, Iran

关键词: MALDI-TOF/TOF;    two-dimensional gel electrophoresis;    somatic embryogenesis;    Crocus sativus L.;    Saffron;   

Others  :  817669
DOI  :  10.1186/1477-5956-10-3

 received in 2011-10-05, accepted in 2012-01-13,  发布年份 2012

【 摘 要 】

Background

Somatic embryogenesis (SE) is a complex biological process that occurs under inductive conditions and causes fully differentiated cells to be reprogrammed to an embryo like state. In order to get a better insight about molecular basis of the SE in Crocus sativus L. and to characterize differentially accumulated proteins during the process, a proteomic study based on two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry has been carried out.

Results

We have compared proteome profiles of non-embryogenic and embryogenic calli with native corm explants. Total soluble proteins were phenol-extracted and loaded on 18 cm IPG strips for the first dimension and 11.5% sodium dodecyl sulfate-polyacrylamide gels for the second dimension. Fifty spots with more than 1.5-fold change in abundance were subjected to mass spectrometry analysis for further characterization. Among them 36 proteins could be identified, which are classified into defense and stress response, protein synthesis and processing, carbohydrate and energy metabolism, secondary metabolism, and nitrogen metabolism.

Conclusion

Our results showed that diverse cellular and molecular processes were affected during somatic to embryogenic transition. Differential proteomic analysis suggests a key role for ascorbate metabolism during early stage of SE, and points to the possible role of ascorbate-glutathione cycle in establishing somatic embryos.

【 授权许可】

   
2012 Sharifi et al; licensee BioMed Central Ltd.

【 图 表 】

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