Immune response to a potyvirus with exposed amino groups available for chemical conjugation

Virology Journal

Abel Gutiérrez-Ortega²  Laura Silva-Rosales³  Mauricio Carrillo-Tripp¹  Gabriela Chavez-Calvillo¹  Pablo César Ortiz-Lazareno⁴  Hernández-Gutiérrez Rodolfo²  Ana Márquez-Aguirre²  Carlos Alberto Manuel-Cabrera² 

[1] Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, Guanajuato 36821, México;Unidad de Biotecnología Médica y Farmacéutica, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Normalistas 800, Colinas de la Normal, Guadalajara, Jalisco 44270, México;Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, km 9.6 Libramiento Norte, Carretera Irapuato-León, Irapuato, Guanajuato 36821, México;División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Independencia, Guadalajara, Jalisco 44340, México

关键词: immune response;    chemical conjugation;    amino groups;    capsid protein;    Tobacco etch virus;   

Others  :  1154817
DOI  :  10.1186/1743-422X-9-75

 received in 2011-10-23, accepted in 2012-03-27,  发布年份 2012

【 摘 要 】

Background

The amino terminus of the tobacco etch virus (TEV) capsid protein is located on the external surface of infectious TEV particles, as proposed by previous studies and an in silico model. The epsilon amino groups on the exposed lysine residues are available for chemical conjugation to any given protein, and can thus act as antigen carriers. The availability of amino groups on the surfaces of TEV particles was determined and the immune response to TEV evaluated.

Results

Using a biotin-tagged molecule that reacts specifically with amino groups, we found that the TEV capsid protein has amino groups on its surface available for coupling to other molecules via crosslinkers. Intraperitoneal TEV was administered to female BALB/c mice, and both their humoral and cellular responses measured. Different IgG isotypes, particularly IgG2a, directed against TEV were induced. In a cell proliferation assay, only spleen cells from vaccinated mice that were stimulated in vitro with TEV showed significant proliferation of CD3⁺/CD4⁺ and CD3⁺/CD8⁺ subpopulations and secreted significant amounts of interferon γ.

Conclusions

TEV has surface amino groups that are available for chemical coupling. TEV induces both humoral and cellular responses when administered alone intraperitoneally to mice. Therefore, TEV should be evaluated as a vaccine adjuvant when chemically coupled to antigens of choice.

【 授权许可】

   
2012 Manuel-Cabrera et al; licensee BioMed Central Ltd.

【 图 表 】

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