A novel bocavirus in canine liver

Virology Journal

Eric Delwart³  Xutao Deng²  Chunlin Wang⁶  Jianming Qiu⁴  Charles Chiu¹  Erik Samayoa¹  Samia N Naccache¹  Lark L Coffey³  Marko Estrada⁵  Christian M Leutenegger⁵  Patricia A Pesavento⁷  Linlin Li³ 

[1] UCSF-Abbott Viral Diagnostics and Discovery Center, CA, USA;Blood Systems Research Institute, San Francisco, CA, USA;Department of Laboratory Medicine, University of California, San Francisco, CA, USA;Department of Microbiology, University of Kansas, KS, USA;IDEXX Reference Laboratories, CA, USA;Stanford Genome Technology Center, Stanford, CA, USA;Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA

关键词: Coinfection;    Episome;    Canine bocavirus 3;   

Others  :  1151830
DOI  :  10.1186/1743-422X-10-54

 received in 2012-12-19, accepted in 2013-01-31,  发布年份 2013

【 摘 要 】

Background

Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease.

Findings

In this study using deep sequencing of enriched viral particles from the liver of a dog with severe hemorrhagic gastroenteritis, necrotizing vasculitis, granulomatous lymphadenitis and anuric renal failure, we identified and characterized a novel bocavirus we named Canine bocavirus 3 (CnBoV3). The three major ORFs of CnBoV3 (NS1, NP1 and VP1) shared less than 60% aa identity with those of other bocaviruses qualifying it as a novel species based on ICTV criteria. Inverse PCR showed the presence of concatemerized or circular forms of the genome in liver.

Conclusions

We genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples. Its role in this animal’s complex disease remains to be determined.

【 授权许可】

   
2013 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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