Dorsoventral patterning of the Xenopus eye involves differential temporal changes in the response of optic stalk and retinal progenitors to Hh signalling

Neural Development

Ying Liu²  William A Harris³  Giuseppina Barsacchi⁴  Rongqiao He²  Giuseppe Lupo¹  Xiumei Wang² 

[1] Department of Chemistry, Sapienza University of Rome, Piazzale A. Moro 5, Rome, 00185, Italy;The State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, Chaoyang District, China;Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK;Dipartimento di Biologia, Unità di Biologia Cellulare e dello Sviluppo, Università di Pisa, SS 12 Abetone e Brennero 4, Pisa, 56127, Italy

关键词: Xenopus;    Hedgehog;    Dorsal retina;    Ventral retina;    Optic stalk;    Optic vesicle;   

Others  :  1145995
DOI  :  10.1186/s13064-015-0035-9

 received in 2014-12-03, accepted in 2015-03-04,  发布年份 2015

【 摘 要 】

Background

Hedgehog (Hh) signals are instrumental to the dorsoventral patterning of the vertebrate eye, promoting optic stalk and ventral retinal fates and repressing dorsal retinal identity. There has been limited analysis, however, of the critical window during which Hh molecules control eye polarity and of the temporal changes in the responsiveness of eye cells to these signals.

Results

In this study, we used pharmacological and molecular tools to perform stage-specific manipulations of Hh signalling in the developing Xenopus eye. In gain-of-function experiments, most of the eye was sensitive to ventralization when the Hh pathway was activated starting from gastrula/neurula stages. During optic vesicle stages, the dorsal eye became resistant to Hh-dependent ventralization, but this pathway could partially upregulate optic stalk markers within the retina. In loss-of-function assays, inhibition of Hh signalling starting from neurula stages caused expansion of the dorsal retina at the expense of the ventral retina and the optic stalk, while the effects of Hh inhibition during optic vesicle stages were limited to the reduction of optic stalk size.

Conclusions

Our results suggest the existence of two competence windows during which the Hh pathway differentially controls patterning of the eye region. In the first window, between the neural plate and the optic vesicle stages, Hh signalling exerts a global influence on eye dorsoventral polarity, contributing to the specification of optic stalk, ventral retina and dorsal retinal domains. In the second window, between optic vesicle and optic cup stages, this pathway plays a more limited role in the maintenance of the optic stalk domain. We speculate that this temporal regulation is important to coordinate dorsoventral patterning with morphogenesis and differentiation processes during eye development.

【 授权许可】

   
2015 Wang et al.; licensee BioMed Central.

【 图 表 】

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