Genetic diversity in Spanish donkey breeds using microsatellite DNA markers

Genetics Selection Evolution

Mariano Gomez¹  Jordi Jordana²  José Aranguren-Méndez³ 

[1] Servicio de Ganadería, Diputación Foral de Bizkaia, Avda. Lehendakari Aguirre, 9, 2°, 48014 Bilbao, Spain;Unitat de Genètica i Millora Animal, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain;Universidad del Zulia, Facultad de Ciencias Veterinarias, Departamento de Producción Animal, Maracaibo 4001-A, Venezuela

关键词: genetic variability;    diversity;    microsatellite;    endangered breed;    donkey;   

Others  :  1094662
DOI  :  10.1186/1297-9686-33-4-433

 received in 2000-11-27, accepted in 2001-04-23,  发布年份 2001

【 摘 要 】

Genetic diversity at 13 equine microsatellite loci was compared in five endangered Spanish donkey breeds: Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine microsatellites used in this study were amplified and were polymorphic in the domestic donkey breeds with the exception of HMS1, which was monomorphic, and ASB2, which failed to amplify. Allele number, frequency distributions and mean heterozygosities were very similar among the Spanish donkey breeds. The unbiased expected heterozygosity (H_E) over all the populations varied between 0.637 and 0.684 in this study. The low G_ST value showed that only 3.6% of the diversity was between breeds (P < 0.01). Significant deviations from Hardy-Weinberg equilibrium were shown for a number of locus-population combinations, except HMS5 that showed agreement in all analysed populations. The cumulative exclusion probability (PE) was 0.999 in each breed, suggesting that the loci would be suitable for donkey parentage testing. The constructed dendrogram from the D_A distance matrix showed little differentiation between Spanish breeds, but great differentiation between them and the Moroccan ass and also with the horse, used as an outgroup. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in domestic donkey populations, which could also be useful for conservation plans.

【 授权许可】

   
2001 INRA, EDP Sciences