TGF-β1 conjugated chitosan collagen hydrogels induce chondrogenic differentiation of human synovium-derived stem cells

Journal of Biological Engineering

Min Lee¹  Denis Evseenko²  Bogyu Choi³  Soyon Kim¹  Brian Lin³  Jinku Kim⁴ 

[1] Department of Bioengineering, University of California, Los Angeles CA 90095, USA;Department of Orthopaedic Surgery, University of California, Los Angeles CA 90095, USA;Division of Advanced Prosthodontics, University of California, Los Angeles CA 90095, USA;Department of Bio and Chemical Engineering, Hongik University, Sejong 339-701, South Korea

关键词: Chondrogenic differentiation;    Synovium-derived stem cells;    Transforming growth factor;    Type II collagen;    Chitosan hydrogels;   

Others  :  1133597
DOI  :  10.1186/1754-1611-9-1

 received in 2014-10-03, accepted in 2014-12-24,  发布年份 2015

【 摘 要 】

Background

Unlike bone tissue, articular cartilage regeneration has not been very successful and has many challenges ahead. We have previously developed injectable hydrogels using photopolymerizable chitosan (MeGC) that supported growth of chondrocytes. In this study, we demonstrate a biofunctional hydrogel for specific use in cartilage regeneration by conjugating transforming growth factor-β1 (TGF-β1), a well-documented chondrogenic factor, to MeGC hydrogels impregnating type II collagen (Col II), one of the major cartilaginous extracellular matrix (ECM) components.

Results

TGF-β1 was delivered from MeGC hydrogels in a controlled manner with reduced burst release by chemically conjugating the protein to MeGC. The hydrogel system did not compromise viability of encapsulated human synovium-derived mesenchymal stem cells (hSMSCs). Col II impregnation and TGF-β1 delivery significantly enhanced cellular aggregation and deposition of cartilaginous ECM by the encapsulated cells, compared with pure MeGC hydrogels.

Conclusions

This study demonstrates successful engineering of a biofunctional hydrogel with a specific microenvironment tailored to promote chondrogenesis. This hydrogel system can provide promising efficacious therapeutics in the treatment of cartilage defects.

【 授权许可】

   
2015 Kim et al.; licensee BioMed Central.

【 图 表 】

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