CD300f immunoreceptor contributes to peripheral nerve regeneration by the modulation of macrophage inflammatory phenotype

Journal of Neuroinflammation

Natalia Lago⁴  Joan Sayós⁵  Ruben López-Vales³  Xavier Navarro³  Isaac Francos-Quijorna³  Maria Luciana Negro-Demontel²  Patricia Solari-Saquieres²  Hugo Peluffo¹ 

[1] Department of Histology and Embryology, Faculty of Medicine, UDELAR, Montevideo, Uruguay;Neuroinflammation and Gene Therapy Laboratory, Institut Pasteur Montevideo, Mataojo 2020, Montevideo, CP 11400, Uruguay;Institute of Neurosciences and Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, and Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Bellaterra, Spain;Neurodegeneration Laboratory, Institut Pasteur Montevideo, Montevideo, Uruguay;Immunobiology Group, CIBBIM-Nanomedicine Program, Hospital Universitari Vall d’Hebron, Institut de Recerca (VHIR), Universitat Autonoma de Barcelona, Barcelona, Spain

关键词: Phagocytosis;    Wallerian degeneration;    Schwann cell;    Macrophage M1/M2 phenotype;    CD300;    Immunoreceptors;    Regeneration;   

Others  :  1227082
DOI  :  10.1186/s12974-015-0364-y

 received in 2015-02-10, accepted in 2015-07-21,  发布年份 2015

【 摘 要 】

Background

It has recently become evident that activating/inhibitory cell surface immune receptors play a critical role in regulating immune and inflammatory processes in the central nervous system (CNS). The immunoreceptor CD300f expressed on monocytes, neutrophils, and mast cells modulates inflammation, phagocytosis, and outcome in models of autoimmune demyelination, allergy, and systemic lupus erythematosus. On the other hand, a finely regulated inflammatory response is essential to induce regeneration after injury to peripheral nerves since hematogenous macrophages, together with resident macrophages and de-differentiated Schwann cells, phagocyte distal axonal and myelin debris in a well-orchestrated inflammatory response. The possible roles and expression of CD300f and its ligands have not been reported under these conditions.

Methods

By using quantitative PCR (QPCR) and CD300f-IgG2a fusion protein, we show the expression of CD300f and its ligands in the normal and crush injured sciatic nerve. The putative role of CD300f in peripheral nerve regeneration was analyzed by blocking receptor-ligand interaction with the same CD300f-IgG2a soluble receptor fusion protein in sciatic nerves of Thy1-YFP-H mice injected at the time of injury. Macrophage M1/M2 polarization phenotype was also analyzed by CD206 and iNOS expression.

Results

We found an upregulation of CD300f mRNA and protein expression after injury. Moreover, the ligands are present in restricted membrane patches of Schwann cells, which remain stable after the lesion. The lesioned sciatic nerves of Thy1-YFP-H mice injected with a single dose of CD300f-IgG2a show long lasting effects on nerve regeneration characterized by a lower number of YFP-positive fibres growing into the tibial nerve after 10 days post lesion (dpl) and a delayed functional recovery when compared to PBS- or IgG2a-administered control groups. Animals treated with CD300f-IgG2a show at 10 dpl higher numbers of macrophages and CD206-positive cells and lower levels of iNOS expression than both control groups. At later time points (28 dpl), increased numbers of macrophages and iNOS expression occur.

Conclusions

Taken together, these results show that the pair CD300f ligand is implicated in Wallerian degeneration and nerve regeneration by modulating both the influx and phenotype of macrophages.

【 授权许可】

   
2015 Peluffo et al.

【 图 表 】

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