Isolation of a novel amylase and lipase-producing Pseudomonas luteola strain: study of amylase production conditions

Lipids in Health and Disease

Imen Fendri³  Néji Gharsallah²  Bassem Khemakhem²  Nour Chaaben¹  Ramzi Miladi¹  Mouna Dammak¹  Mouna Jrad²  Lamia Khannous³ 

[1] Département de génie biologique, Ecole Nationale d’Ingénieurs de Sfax, Université de Sfax, Sfax, Tunisia;Laboratoire de Biotechnologie des Plantes, Faculté des Sciences de Sfax, Université de Sfax, Sfax, Tunisia;Faculté des Sciences de Sfax, Unité de recherche Toxicologie- Microbiologie Environnementale et Santé, Université de, Sfax, Tunisia

关键词: Box Behnken Design;    Lipase;    Amylase;    Pseudomonas luteola;   

Others  :  814047
DOI  :  10.1186/1476-511X-13-9

 received in 2013-08-23, accepted in 2013-12-28,  发布年份 2014

【 摘 要 】

An amylase and lipase producing bacterium (strain C2) was enriched and isolated from soil regularly contaminated with olive washing wastewater in Sfax, Tunisia. Cell was aerobic, mesophilic, Gram-negative, motile, non-sporulating bacterium, capable of growing optimally at pH 7 and 30°C and tolerated maximally 10% (W/V) NaCl. The predominant fatty acids were found to be C_18:1ω7c (32.8%), C_16:1ω7c (27.3%) and C_16:0 (23.1%). Phylogenetic analysis of the 16S rRNA gene revealed that this strain belonging to the genus Pseudomonas. Strain C2 was found to be closely related to Pseudomonas luteola with more than 99% of similarity. Amylase optimization extraction was carried out using Box Behnken Design (BBD). Its maximal activity was found when the pH and temperature ranged from 5.5 to 6.5 and from 33 to 37°C, respectively. Under these conditions, amylase activity was found to be about 9.48 U/ml.

【 授权许可】

   
2014 Khannous et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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