Gene expression analysis in response to osmotic stimuli in the intervertebral disc with DNA microarray

European Journal of Medical Research

Feng Zhang¹  Liqun Duan¹  Rui He¹  Xiang Xu¹  Yefeng Hu¹  Qichun Zhao¹  Xifu Shang¹  Xu Li¹  Wenzhi Zhang¹ 

[1] Department of Orthopaedics, Anhui Provincial Hospital, No. 17, Road Lujiang, Hefei 230001, China

关键词: Osmotic stimuli;    Osmotic pressure;    Top-scoring pair;    Intervertebral disc;   

Others  :  817681
DOI  :  10.1186/2047-783X-18-62

 received in 2013-06-27, accepted in 2013-12-06,  发布年份 2013

【 摘 要 】

Background

Intervertebral disc (IVD) cells experience a broad range of physicochemical stimuli under physiologic conditions, including alterations in their osmotic environment. At present, the molecular mechanisms underlying osmotic regulation in IVD cells are poorly understood. This study aims to screen genes affected by changes in osmotic pressure in cells of subjects aged 29 to 63 years old, with top-scoring pair (TSP) method.

Methods

Gene expression data set GSE1648 was downloaded from Gene Expression Omnibus database, including four hyper-osmotic stimuli samples, four iso-osmotic stimuli samples, and three hypo-osmotic stimuli samples. A novel, simple method, referred to as the TSP, was used in this study. Through this method, there was no need to perform data normalization and transformation before data analysis.

Results

A total of five pairs of genes ((CYP2A6, FNTB), (PRPF8, TARDBP), (RPS5, OAZ1), (SLC25A3, NPM1) and (CBX3, SRSF9)) were selected based on the TSP method. We inferred that all these genes might play important roles in response to osmotic stimuli and age in IVD cells. Additionally, hyper-osmotic and iso-osmotic stimuli conditions were adverse factors for IVD cells.

Conclusions

We anticipate that our results will provide new thoughts and methods for the study of IVD disease.

【 授权许可】

   
2013 Zhang et al.; licensee BioMed Central Ltd.

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