Up-regulation of endoplasmic reticulum stress induced genes of the unfolded protein response in the liver of periparturient dairy cows

BMC Veterinary Research

Klaus Eder²  Frieder J Schwarz¹  Robert Ringseis²  Gloria Schlegel²  Denise K Gessner² 

[1] Chair of Animal Nutrition, Center of Life and Food Sciences Weihenstephan, Technische Universität München, Liesel-Beckmann-Strasse 6, Freising, Weihenstephan D-85350, Germany;Institute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, Giessen D-35392, Germany

关键词: Unfolded protein response;    ER stress;    Liver;    Dairy cow;   

Others  :  1119324
DOI  :  10.1186/1746-6148-10-46

 received in 2013-07-10, accepted in 2014-02-14,  发布年份 2014

【 摘 要 】

Background

In dairy cows, the periparturient phase is a stressful period, which is commonly associated with strong metabolic adaptations and the development of pathophysiologic conditions and disorders. Some of the symptoms occurring in the liver, such as the development of fatty liver, are similar to those observed under the condition of endoplasmic reticulum (ER) stress. Therefore, we hypothesized, that in the liver of dairy cows ER stress is induced during the periparturient phase, which in turn leads to an induction of the unfolded protein response (UPR). In order to investigate this hypothesis, we determined relative mRNA concentrations of 14 genes of the ER stress-induced UPR in liver biopsy samples of 13 dairy cows at 3 wk antepartum and 1, 5 and 14 wk postpartum.

Results

We found, that the mRNA concentrations of 13 out of the 14 genes involved in the UPR in the liver were significantly increased (1.9 to 4.0 fold) at 1 wk postpartum compared to 3 wk antepartum. From 1 wk postpartum to later lactation, mRNA concentrations of all the genes considered were declining. Moreover, at 1 wk postpartum, mRNA concentration of the spliced variant of XBP1 was increased in comparison to 3 wk antepartum, indicating that splicing of XBP1 – a hallmark of ER stress - was induced following the onset of lactation.

Conclusion

The present study reveals, that ER stress might be induced during the periparturient phase in the liver of dairy cows. We assume that the ER stress-induced UPR might contribute to the pathophysiologic conditions commonly observed in the liver of periparturient cows, such as the development of fatty liver, ketosis or inflammation.

【 授权许可】

   
2014 Gessner et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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