The effect of dietary tryptophan levels on oxidative stress of liver induced by diquat in weaned piglets

Journal of Animal Science and Biotechnology

Daiwen Chen¹  Quyuan Wang¹  Jie Yu¹  Ping Zheng¹  Jun He¹  Bing Yu¹  Mei Lv¹  Xiangbing Mao¹ 

[1] Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Education, Ya’an 625014, People’s Republic of China

关键词: Weaned piglets;    Tryptophan;    Oxidative stress of livers;    Diquat;    Antioxidant capacity;   

Others  :  1135855
DOI  :  10.1186/2049-1891-5-49

 received in 2014-06-07, accepted in 2014-10-27,  发布年份 2014

【 摘 要 】

Oxidative stress can induce abnormal tryptophan metabolism. The present study was mainly conducted to determine the effect of dietary tryptophan levels on oxidative stress in the liver of weaned pigs challenged by diquat. A total of 36 PIC piglets weaned at 21 days of age were randomly allotted to 1 of 3 diets containing dietary tryptophan levels of 0.18, 0.30, and 0.45% for 14 d. On day 8, the piglets were injected intraperitoneally with sterile 0.9% NaCl solution or diquat (10 mg/kg body weight). During the first 7 d of trial, increasing dietary tryptophan levels enhanced average daily gain (P = 0.09) and average daily feed intake (P = 0.08), and decreased the feed efficiency (P < 0.05) of piglets. The growth performance was decreased by diquat injection (P < 0.05). Diquat injection also decreased the activities of the superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the plasma and liver (P < 0.05), increased plasma malondialdehyde (MDA) (P < 0.05) and urea nitrogen (P < 0.05) concentrations, and enhanced MDA concentration (P = 0.09) and tryptophan 2,3-dioxygenase (TDO) activity (P = 0.07) in liver of piglets. Increasing dietary tryptophan levels could attenuate the effects of diquat injection on the MDA (P = 0.06) concentration and the activities of SOD (P = 0.09) and GPx (P = 0.05) of the liver, and plasma urea nitrogen (P = 0.06) concentration in the piglet. There was a synergistic role for increasing TDO activity in the liver between dietary tryptophan levels and diquat injection (P < 0.05). These results suggest that increasing dietary tryptophan levels could attenuate the oxidative stress of the liver in weaned piglets intraperitoneally injected with diquat via enhancing the antioxidant capacity.

【 授权许可】

   
2014 Mao et al.; licensee BioMed Central Ltd.

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