Myosin VI contributes to maintaining epithelial barrier function

Journal of Biomedical Science

Xiu-Ying Wu¹  Ling-Xin Meng¹  Tao Hong¹  Xiao-Lei Wu¹  Jia Jia¹  Xing-Mao Wu¹  Yu-Wei Liao¹ 

[1] Department of Anesthesiology, Shengjing Hospital, China Medical University, 36 Sanhao St, Shenyang 110004, China

关键词: Myosin;    Barrier function;    Epithelium;    Lysosome;    Endosome;   

Others  :  823295
DOI  :  10.1186/1423-0127-20-68

 received in 2013-06-20, accepted in 2013-09-10,  发布年份 2013

【 摘 要 】

Background

Epithelial barrier dysfunction is associated with the pathogenesis of a number of immune inflammations; the etiology is not fully understood. The fusion of endosome/lysosome is a critical process in the degradation of endocytic antigens in epithelial cells. Recent reports indicate that myosin VI (myo6) is involved in the activities of endosomes. The present study aims to investigate the role of myo6 in epithelial barrier dysfunction.

Results

The endosome accumulation was observed in myo6-deficient Rmcs. More than 80% endosomes were fused with lysosomes in naïve Rmcs while less than 30% endosomes were fused with lysosomes in the myo6-deficient Rmcs. The myo6-deficient Rmc monolayers showed high permeability to a macromolecular antigen, ovalbumin, the latter still conserved the antigenicity, which induced strong T cell activation.

Conclusions

We conclude that myo6 plays a critical role in the fusion of endosome/lysosome in Rmc epithelial cells. Deficiency of myo6 compromises the epithelial barrier function.

【 授权许可】

   
2013 Liao et al.; licensee BioMed Central Ltd.

【 图 表 】

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