Effects in cigarette smoke stimulated bronchial epithelial cells of a corticosteroid entrapped into nanostructured lipid carriers

Journal of Nanobiotechnology

Elisabetta Pace³  Mark Gjomarkaj³  Chiara Botto¹  Gaetano Giammona¹  Gennara Cavallaro¹  Stefania Gerbino³  Serena Di Vincenzo³  Maria Ferraro³  Maria Luisa Bondì² 

[1] Laboratory of Biocompatible Polymers-Dipartimento di Scienze e Tecnologie, Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università di Palermo -via Archirafi, Palermo, 32-90123, Italy;Istituto per lo Studio dei Materiali Nanostrutturati- U.O.S. di Palermo-Consiglio Nazionale delle Ricerche-via Ugo La Malfa, Palermo, 153 90146, Italy;Istituto di Biomedicina e Immunologia Molecolare-Consiglio Nazionale delle Ricerche – via Ugo La Malfa, Palermo, 153 90146, Italy

关键词: Asthma;    Chronic obstructive pulmonary disease;    Airway epithelial cell;    Cigarette smoke;    Fluticasone propionate;    Corticosteroid;    Nanostructured lipid carriers;   

Others  :  1139417
DOI  :  10.1186/s12951-014-0046-4

 received in 2014-09-02, accepted in 2014-10-23,  发布年份 2014

【 摘 要 】

Background

Nanomedicine studies have showed a great potential for drug delivery into the lung. In this manuscript nanostructured lipid carriers (NLC) containing Fluticasone propionate (FP) were prepared and their biocompatibility and effects in a human bronchial epithelial cell line (16-HBE) stimulated with cigarette smoke extracts (CSE) were tested.

Results

Biocompatibility studies showed that the NLC did not induce cell necrosis or apoptosis. Moreover, it was confirmed that CSE increased intracellular ROS production and TLR4 expression in bronchial epithelial cells and that FP-loaded NLC were more effective than free drug in modulating these processes. Finally, the nanoparticles increased GSH levels improving cell protection against oxidative stress.

Conclusions

The present study shows that NLC may be considered a promising strategy to improve corticosteroid mediated effects in cellular models associated to corticosteroid resistance. The NLC containing FP can be considered good systems for dosage forms useful for increasing the effectiveness of fluticasone decreasing its side effects.

【 授权许可】

   
2014 Bondì et al.; licensee BioMed Central Ltd.

【 图 表 】

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