Mir-208 promotes cell proliferation by repressing SOX6 expression in human esophageal squamous cell carcinoma

Journal of Translational Medicine

Jianyong Yang⁴  Yong Gu³  Shiqiu Xiong¹  Wei Chen⁴  Junwei Ou³  Liangshuai Liu⁴  Bing Zhang⁴  Dayong Zheng²  Heping Li⁵ 

[1] Department of Biochemistry, University of Leicester, Leicester, UK;Department of Oncology, Nanfang Hosptial, Southern Medical University, Guangzhou 510515, P.R. China;Department of Thoracic Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P.R. China;Department of Medical Imaging, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P.R. China;Department of Oncology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, P.R. China

关键词: Proliferation;    Esophageal squamous cell carcinoma;    SOX6;    miR-208;   

Others  :  1148685
DOI  :  10.1186/1479-5876-12-196

 received in 2014-01-05, accepted in 2014-07-01,  发布年份 2014

【 摘 要 】

Background

Esophageal squamous cell carcinoma (ESCC) is the major histological type of esophageal cancer in developing countries. The prognosis and survival rate of ESCC are very poor. Recently, microRNAs (miRNAs) have emerged as important regulators of cancer cell biological processes. To better understanding the molecular mechanisms by which they regulate the behavior of cancer cells is needed.

Methods

The expression of miR-208 was examined in ESCC cell lines and tumor tissues by real-time PCR. Proliferation capability of ESCC cells upon regulation of miR-208 expression was detected by MTT assay, colony formation assay, anchorage-independent growth ability assay and flow cytometry analysis. The target of miR-208 was determined by western blotting analysis, luciferase reporter assay and real-time PCR.

Results

miR-208 was upregulated in ESCC cell lines and tissues. Overexpression of miR-208 in ESCC cells increased cell proliferation, tumorigenicity and cell cycle progression, whereas inhibition of miR-208 reduced cells proliferation, tumorigenicity and cell cycle progression. Additionally, SOX6 was identified as a direct target of miR-208. Ectopic expression of miR-208 led to downregulation of SOX6 protein, which resulted in the downregulation of p21, upregulation of cyclin D1 and phosphorylation of Rb.

Conclusions

These results suggest that miR-208 represents a potential onco-miR and participates in ESCC carcinogenesis by suppressing SOX6 expression.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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