Comparative analysis of proteomic profiles between endometrial caruncular and intercaruncular areas in ewes during the peri-implantation period

Journal of Animal Science and Biotechnology

Lei An³  Jianhui Tian³  Zhonghong Wu²  Min Guo³  Rui Wang³  Haichao Zhao³  Kai Miao³  Zhuocheng Hou¹  Chao Wang³  Yang Wang³ 

[1] National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, China Agricultural University, Beijing 100193, China;State Key Laboratory of Animal Nutrition, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China;Ministry of Agriculture Key Laboratory of Animal Genetics, Breeding and Reproduction, National engineering laboratory for animal breeding, College of Animal Sciences and Technology, China Agricultural University, No.2 Yuanmingyuan Xi Lu, Haidian, Beijing 100193, China

关键词: Intercaruncular areas;    Implantation;    Ewe;    Endometrium;    Caruncular areas;   

Others  :  805099
DOI  :  10.1186/2049-1891-4-39

 received in 2013-05-16, accepted in 2013-09-23,  发布年份 2013

【 摘 要 】

The endometrium of sheep consists of plenty of raised aglandular areas called caruncular (C), and intensely glandular intercaruncular areas (IC). In order to better understand the endometrium involved mechanisms of implantation, we used LC-MS/MS technique to profile the proteome of ovine endometrial C areas and IC areas separately during the peri-implantation period, and then compared the proteomic profiles between these two areas. We successfully detected 1740 and 1813 proteins in C areas and IC areas respectively. By comparing the proteome of these two areas, we found 170 differentially expressed proteins (DEPs) (P < 0.05), functional bioinformatics analysis showed these DEPs were mainly involved in growth and remodeling of endometrial tissue, cell adhesion and protein transport, and so on. Our study, for the first time, provided a proteomic reference for elucidating the differences between C and IC areas, as an integrated function unit respectively, during the peri-implantation period. The results could help us to better understand the implantation in the ewes. In addition, we established a relatively detailed protein database of ovine endometrium, which provide a unique reference for further studies.

【 授权许可】

   
2013 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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