Interaction between cytokine gene polymorphisms and the effect of physical exercise on clinical and inflammatory parameters in older women: study protocol for a randomized controlled trial

Trials

Leani SM Pereira¹  Antônio L Teixeira³  Danielle AG Pereira²  Rosângela C Dias²  Fabianna Jesus-Moraleida²  Daniela MC Anjos²  Aline S Miranda³  Diogo C Felício²  Alexandra M Assumpção²  Elvis CC Mateo³  Bárbara Z Queiroz²  Daniele S Pereira² 

[1] Departamento de Fisioterapia, Universidade Federal de Minas Gerais / UFMG, Av. Antônio Carlos, 6627, CEP 31270-901, Belo Horizonte, MG, Brazil;Department of Physical Therapy, Graduate Program in Rehabilitation Sciences, School of Physical Education, Physical Therapy and Occupational Therapy, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;Department of Internal Medicine, School of Medicine, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil

关键词: BDNF;    Physical performance;    Exercise;    Older adult;    Cytokines;    Polymorphism;   

Others  :  1095416
DOI  :  10.1186/1745-6215-13-134

 received in 2012-01-17, accepted in 2012-07-04,  发布年份 2012

【 摘 要 】

Background

Aging is associated with chronic low-grade inflammatory activity with an elevation of cytokine levels. An association between regular physical activity and reduction of blood levels of anti-inflammatory cytokines is demonstrated in the literature pointing to an anti-inflammatory effect related to exercise. However, there is no consensus regarding which type of exercise and which parameters are the most appropriate to influence inflammatory markers. Evidence indicates that the single nucleotide polymorphism (SNP) can influence the synthesis of those cytokines affecting their production.

Methods/Design

The design of this study is a randomized controlled trial. The aim of this study is to investigate the interaction between the cytokine genes SNP and the effect of physical activity on older women. The main outcomes are: serum levels of sTNFR-1, sTNFR-2, interleukin (IL)-6, IL-10, measured by the ELISA method; genotyping of tumor necrosis factor- (TNF)-alpha (rs1800629), IL6 (rs1800795), IL10 (rs1800896) by the TaqMan Method (Applied Biosystems, Foster City, CA, USA); and physical performance assessed by Timed Up and Go and 10-Meter Walk Tests. Secondary outcomes include: Geriatric Depression Scale, Perceived Stress Scaleand aerobic capacity, assessed by the six-minute walk; and lower limb muscle strength, using an isokinetic dinamometer (Biodex Medical Systems, Inc., Shirley, NY,USA). Both exercise protocols will be performed three times a week for 10 weeks, 30 sessions in total.

Discussion

Investigating the interaction between genetic factors and exercise effects of both protocols of exercise on the levels of inflammatory cytokine levels can contribute to guide clinical practice related to treatment and prevention of functional changes due to chronic inflammatory activity in older adults. This approach could develop new perspectives on preventive and treatment proposals in physical therapy and in the management of the older patient.

Trial registration

(ReBEC) RBR9v9cwf

【 授权许可】

   
2012 Pereira et al.; licensee BioMed Central Ltd.

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