Effects of

Journal of Animal Science and Biotechnology

Qingyan Jiang²  Yongliang Zhang²  Qianyun Xi²  Ping Gao²  Lina Wang²  Xiaotong Zhu²  Gang Shu²  Jianjian Yu²  Shengfeng Chen²  Paul Khondowe¹  Songbo Wang² 

[1] School of Natural Sciences, Department of Biological Sciences, University of Zambia, P.O. Box 32379, Lusaka, Zambia;College of Animal Sciences, South China Agricultural University, Guangzhou, 510642, P. R. China

关键词: L-glutamate;    L-leucine;    Intracerebroventricular (ICV);    Hypothalamus;    Feed intake;    Broiler chicks;   

Others  :  813435
DOI  :  10.1186/2049-1891-3-27

 received in 2012-02-22, accepted in 2012-08-17,  发布年份 2012

【 摘 要 】

Feed intake control is vital to ensuring optimal nutrition and achieving full potential for growth and development in poultry. The aim of the present study was to investigate the effects of L-leucine, L-glutamate, L-tryptophan and L-arginine on feed intake and the mRNA expression levels of hypothalamic Neuropeptide involved in feed intake regulation in broiler chicks. Leucine, glutamate, tryptophan or arginine was intra-cerebroventricularly (ICV) administrated to 4d-old broiler chicks respectively and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of Neuropeptide Y (NPY), agouti related protein (AgRP), pro-opiomelanocortin (POMC), melanocortin receptor 4 (MC4R) and corticotrophin releasing factor (CRF). Our results showed that ICV administration of L-leucine (0.15 or 1.5  μmol) significantly (P < 0.05) increased feed intake up to 2 h post-administration period and elevated both hypothalamic NPY and AgRP mRNA expression levels. In contrast, ICV administration of L-glutamate (1.6  μmol) significantly (P < 0.05) decreased feed intake 0.25, 0.5 and 2 h post-injection, and increased hypothalamic CRF and MC4R mRNA expression levels. Meanwhile, both L-tryptophan (10 or 100  μg) and L-arginine (20 or 200  μg) had no significant effect on feed intake. These findings suggested that L-leucine and L-glutamate could act within the hypothalamus to influence food intake, and that both orexigenic and anorexigenic Neuropeptide genes might contribute directly to these effects.

【 授权许可】

   
2012 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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