Transauricular embolization of the rabbit coronary artery for experimental myocardial infarction: comparison of a minimally invasive closed-chest model with open-chest surgery

Journal of Cardiothoracic Surgery

Dimitris Siablis⁵  Dimitrios Dougenis⁴  Athanassios Diamantopoulos⁵  Fevronia Kolonitsiou³  Dimitris Karnabatidis⁵  Vassiliki Bravou¹  Efstratios Koletsis⁴  Sofoklis Mitsos²  Konstantinos Katsanos⁵ 

[1] Department of Anatomy, Patras University, School of Medicine, 26504, Rion, Greece;Department of Cardiothoracic Surgery, Onassion Cardiac Surgery Center, Athens, Greece;Department of Microbiology, Patras University Hospital, School of Medicine, 26504, Rion, Greece;Department of Cardiothoracic Surgery, Patras University Hospital, School of Medicine, 26504, Rion, Greece;Department of Interventional Radiology, Patras University Hospital, School of Medicine, 26504, Rion, Greece

关键词: ligation;    embolization;    myocardial ischemia;    experimental;    animal study;    left anterior descending;    transcatheter;    heart;    coil;    endovascular;   

Others  :  1153418
DOI  :  10.1186/1749-8090-7-16

 received in 2011-10-19, accepted in 2012-02-13,  发布年份 2012

【 摘 要 】

Introduction

To date, most animal studies of myocardial ischemia have used open-chest models with direct surgical coronary artery ligation. We aimed to develop a novel, percutaneous, minimally-invasive, closed-chest model of experimental myocardial infarction (EMI) in the New Zealand White rabbit and compare it with the standard open-chest surgical model in order to minimize local and systemic side-effects of major surgery.

Methods

New Zealand White rabbits were handled in conformity with the "Guide for the Care and Use of Laboratory Animals" and underwent EMI under intravenous anesthesia. Group A underwent EMI with an open-chest method involving surgical tracheostomy, a mini median sternotomy incision and left anterior descending (LAD) coronary artery ligation with a plain suture, whereas Group B underwent EMI with a closed-chest method involving fluoroscopy-guided percutaneous transauricular intra-arterial access, superselective LAD catheterization and distal coronary embolization with a micro-coil. Electrocardiography (ECG), cardiac enzymes and transcatheter left ventricular end-diastolic pressure (LVEDP) measurements were recorded. Surviving animals were euthanized after 4 weeks and the hearts were harvested for Hematoxylin-eosin and Masson-trichrome staining.

Results

In total, 38 subjects underwent EMI with a surgical (n = 17) or endovascular (n = 21) approach. ST-segment elevation (1.90 ± 0.71 mm) occurred sharply after surgical LAD ligation compared to progressive ST elevation (2.01 ± 0.84 mm;p = 0.68) within 15-20 min after LAD micro-coil embolization. Increase of troponin and other cardiac enzymes, abnormal ischemic Q waves and LVEDP changes were recorded in both groups without any significant differences (p > 0.05). Infarct area was similar in both models (0.86 ± 0.35 cm in the surgical group vs. 0.92 ± 0.54 cm in the percutaneous group;p = 0.68).

Conclusion

The proposed model of transauricular coronary coil embolization avoids thoracotomy and major surgery and may be an equally reliable and reproducible platform for the experimental study of myocardial ischemia.

【 授权许可】

   
2012 Katsanos et al; licensee BioMed Central Ltd.

【 图 表 】

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