期刊论文详细信息
Clinical Epigenetics
Nucleosome positioning is unaltered at MLH1 splice site mutations in cells derived from Lynch syndrome patients
Robyn L Ward2  Bryony A Thompson1  Andrea C Nunez2  Luke B Hesson2  Mathew A Sloane2 
[1] Department of Genetics and Computational Biology, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia;Adult Cancer Program, Lowy Cancer Research Centre and Prince of Wales Clinical School, University of New South Wales, Sydney, NSW, Australia
关键词: Exon;    Donor;    Acceptor;    Splicing;    Splice site;    Nucleosome;    Colorectal cancer;    Lynch syndrome;   
Others  :  1092758
DOI  :  10.1186/s13148-014-0032-6
 received in 2014-07-04, accepted in 2014-11-28,  发布年份 2014
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【 摘 要 】

Background

Splicing is more efficient when coupled with transcription and it has been proposed that nucleosomes enriched in exons are important for splice site recognition. Lynch syndrome is a familial cancer syndrome that can be caused by the autosomal dominant inheritance of splice site mutations in the MutL homolog 1 (MLH1) gene. To better understand the role of nucleosomes in splicing, we used MLH1 splice site mutations in Lynch syndrome cases as a model to investigate if abnormal splicing was associated with altered nucleosome positioning at exon-intron boundaries.

Findings

Nucleosome Occupancy and Methylome sequencing (NOMe-seq) was used to determine the allele-specific positioning of nucleosomes around heterozygous splice site mutations in lymphoblastoid cells lines (LCLs) derived from six Lynch syndrome patients. These mutations were previously shown to cause exon skipping in five of the six patients. Allele-specific high-resolution nucleosome mapping across exons and exon-intron boundaries revealed high levels of nucleosomes across all regions examined. Alleles containing donor or acceptor splice site mutations showed no consistent alteration in nucleosome positioning or occupancy.

Conclusion

Nucleosomes were enriched at MLH1 exons in LCLs derived from Lynch syndrome patients, and in this model system the positioning of nucleosomes was unaltered at exon-intron boundaries containing splice site mutations. Thus, these splice site mutations alone do not significantly change the local organisation of nucleosomes.

【 授权许可】

   
2014 Sloane et al.; licensee BioMed Central Ltd.

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