Morphology of ovaries in laron dwarf mice, with low circulating plasma levels of insulin-like growth factor-1 (IGF-1), and in bovine GH-transgenic mice, with high circulating plasma levels of IGF-1

Journal of Ovarian Research

Mariusz Z Ratajczak⁴  Magda Kucia⁴  Andrzej Bartke¹  John J Kopchick²  Wojciech Głąbowski³  Katarzyna Piotrowska⁵  Maria Laszczyńska⁶  Sylwia Słuczanowska-Głąbowska⁵ 

[1] Geriatrics Research, Departments of Internal Medicine and Physiology, Southern Illinois University School of Medicine, Springfield, IL, USA;Edison Biotechnology Institute and Department of Biomedical Sciences, College of Osteopathic Medicine, Ohio University, Athens, OH, USA;Department of Histology and Embryology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111, Szczecin, Poland;Stem Cell Biology Program, James Graham Brown Cancer Center, University of Louisville, Louisville, Ky, USA;Department of Physiology, Pomeranian Medical University, Powstańców Wielkopolskich 72, 70-111, Szczecin, Poland;Department of Histology and Developmental Biology, Pomeranian Medical University, Żołnierska 48, 71-210, Szczecin, Poland

关键词: Aging;    Insulin-like growth factor-1;    Growth hormone;    Bovine growth hormone transgenic mouse;    Laron dwarf mouse;    Murine ovary;   

Others  :  824289
DOI  :  10.1186/1757-2215-5-18

 received in 2012-05-06, accepted in 2012-07-02,  发布年份 2012

【 摘 要 】

Background

It is well known that somatotrophic/insulin signaling affects lifespan in experimental animals, and one of the signs of aging is progressive gonadal dysfunction.

Methods

To study the effects of insulin-like growth factor-1 (IGF-1) plasma level on ovaries, we analyzed ovaries isolated from 2-year-old growth hormone receptor knockout (GHR-KO) Laron dwarf mice, with low circulating plasma levels of IGF-1, and 6-month-old bovine growth hormone transgenic (bGHTg) mice, with high circulating plasma levels of IGF-1. The ages of the Laron dwarf mutants employed in our studies were selected based on their overall survival (up to _~ 4 years for Laron dwarf mice and _~ 1 year for bGHTg mice).

Results

Morphological analysis of the ovaries of mice that reached _~50% of their maximal life span revealed a lower biological age for the ovaries isolated from 2-year-old Laron dwarf mice than their normal-lifespan wild type littermates. By contrast, the ovarian morphology of increased in size 6 month old bGHTg mice was generally normal.

Conclusion

Ovaries isolated from 2-year-old Laron dwarf mice exhibit a lower biological age compared with ovaries from normal WT littermates at the same age. At the same time, no morphological features of accelerated aging were found in 0.5-year-old bGHTg mice compared with ovaries from normal the same age-matched WT littermates.

【 授权许可】

   
2012 Sluczanowska-Glabowska et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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