Hypolipidemic effect of gypenosides in experimentally induced hypercholesterolemic rats

Lipids in Health and Disease

Qing-Hua Jiang¹  Jun Yang¹  Yue-Hui Yang¹ 

[1] Pharmaceutical department, Shengjing Hospital, China Medical University, 36 Sanhao Road, Shenyang 110004, China

关键词: Lipid metabolism;    Hyperlipidemia;    Gynosaponins;   

Others  :  829471
DOI  :  10.1186/1476-511X-12-154

 received in 2013-09-13, accepted in 2013-10-23,  发布年份 2013

【 摘 要 】

Background

To investigate the anti-hyperlipidemic effect of gynosaponins (GPs) in hyperlipidemic rats induced by high lipid diet.

Methods

Animal model of hyperlipidemia was established by high-fat and high-cholesterol diet. Rats were randomly divided into 6 groups, except the normal and model groups, rats in GPs groups were daily administered intragastrically with GPs (50, 100, and 200 mg/kg), and rats in simvastatin group were daily administered intragastrically with simvastatin (10 mg/kg). It was measured that the contents of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), total cholesterol (TC), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) in the serum, TG and TC in the liver during this experiment, respectively. The left lobe of liver was observed by histopathological staining, and the immunohistochemical staining was used to observe the effects on the effect of GPs on liver functions.

Results

Compared with the model group, GPs groups could remarkably decrease the content of lipids, GSH-Px, SOD, CAT and MDA in the serum and TC and TG in the liver of the hyperlipidemic rats. The pathomorphological results of hepatic tissue showed that fatty degeneration and inflammatory reaction of GPs groups were lightened compared with the model group.

Conclusions

The results show that GPs has good effects on the treatment of hyperlipidemia induced by high lipid diet in rats. The possible anti-hyperlipidemia mechanism maybe those GPs can regulate the disorder of lipid metabolism as well as ameliorate hepatic function.

【 授权许可】

   
2013 Yang et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

【 参考文献 】

• [1]Smith SC, Jackson R, Pearson TA: Principles for national and regional guidelines on cardiovascular disease prevention: a scientific statement from the world health and stroke forum. Circulation 2004, 109:3112-3121.
• [2]Lin J, Lin C, Chiu H, Yang J, Lee S: Evaluation of the antfnflammatory and liver-protective effects of Anoectochilus formosanus, Ganoderma lucidum and Gynostemma pentaphyllum in rats. Am J Chin Med 1992, 21:59-69.
• [3]Chou SC, Chen KW, Hwang JS: The add-on effects of Gynostemma pentaphyllum on nonalcoholic fatty liver disease. Altern Ther Health Med 2006, 12:34-39.
• [4]Piao XL, Wu Q: Progressive studies on Gynostemma pentaphyllum. Lishizhen medicine material medica research 2010, 21:1758-1760.
• [5]Huang TH, Li Y, Razmovski-Naumovsi V: Gypenoside XLIX isolated from Gynostemma pentaphyllum inhibits nuclear factor-kappa p activation via a PPAR-alpha-dependent pathway. J Biomed Sci 2006, 13:535-548.
• [6]Kimura Y, Okuda H, Arichi S, Takemoto T: Effects of crude Gynostemma pentaphyllum on lipid metabolism. Shoyakugaku Zasshi 1983, 37:272.
• [7]Brown MS, Goldstein JL: Suppression of 3-hydroxy-3-methylglutaryl coenzyme a reductase activity and inhibition of growth of human fibroblasts by 7-ketocholesterol. J Biol Chem 1974, 249:7306-7314.
• [8]Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
• [9]Rubins HB, Robins SJ, Collins D: Gemfibrozil for the secondary prevention of coronary heart disease in men with low levels of high-density lipoprotein cholesterol. N Engl J Med 1999, 341:410-418.
• [10]Qi G, Zhang L, Li CL: Influence of Gypenoside on serum lipoprotein and atherosclerosis in Hyperlipidaemia animals. China Journal of Chinese Materia Medica 1996, 21:562-564.
• [11]Perez Y, Oyarzabal A, Mas R: Protective effect of D-002, a mixture of beeswax alcohols, against indomethacin-induced gastric ulcers and mechanism of action. J Nat Med 2012, 67:182-189.
• [12]Wang JQ, Zou YH, Huang C: Protective effects of tiopronin against high fat dietinduced non-alcoholic steatohepatitis in rats. Acta Pharmacol Sin 2012, 2012:1-7.
• [13]Leopold JA, Loscalzo J: Oxidative mechanisms and atherothrombotic cardiovascular disease: drug discovery today: therapeutic strategies. Cardiovascular diseases 2008, 5:5-13.
• [14]Zhang ZW, Wang QH, Zhang JL: Effect s of oxidative stress on immunos uppression induced by selenium deficiency in chickens. Biol Trace Elem Res 2012, 149:352-361.
• [15]Megalli S, Aktan F, Davies NM, Roufogalis BD: Phytopreventative anti-Hyperlipidemic effects of Gynostemma pentaphyllum in rats. J Pharm Pharmaceut Sci 2005, 8:507-515.
• [16]Megalli S, Davies NM, Roufogalis BD: Anti-Hyperlipidemic and Hypoglycemic effects of Gynostemma pentaphyllum in the Zucker fatty rat. J Pharm Pharmaceut Sci 2006, 9:281-291.
• [17]Li L, Lau B: Protection of vascular endothelial cells from hydrogen peroxide-induced oxidant injury by gypenosides, Saponins of Gynostemma pentaphyllum. Phytother Res 1992, 7:299-304.