ZBTB33 binds unmethylated regions of the genome associated with actively expressed genes

Epigenetics & Chromatin

Peggy J Farnham³  Victor X Jin²  Zhenqing Ye²  Yao Wang²  Lijing Yao³  Adam Blattler¹ 

[1] Genetics Graduate Group, University of California-Davis, Davis, CA, 95616, USA;Department of Biomedical Informatics, The Ohio State University, Columbus, OH, 43210, USA;Department of Biochemistry & Molecular Biology, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA, 90089, USA

关键词: Transcriptional regulation;    Epigenetics;    Transcription factor binding;    Histone modifications;    Zinc finger proteins;    DNA methylation;   

Others  :  811000
DOI  :  10.1186/1756-8935-6-13

 received in 2013-03-18, accepted in 2013-04-16,  发布年份 2013

【 摘 要 】

Background

DNA methylation and repressive histone modifications cooperate to silence promoters. One mechanism by which regions of methylated DNA could acquire repressive histone modifications is via methyl DNA-binding transcription factors. The zinc finger protein ZBTB33 (also known as Kaiso) has been shown in vitro to bind preferentially to methylated DNA and to interact with the SMRT/NCoR histone deacetylase complexes. We have performed bioinformatic analyses of Kaiso ChIP-seq and DNA methylation datasets to test a model whereby binding of Kaiso to methylated CpGs leads to loss of acetylated histones at target promoters.

Results

Our results suggest that, contrary to expectations, Kaiso does not bind to methylated DNA in vivo but instead binds to highly active promoters that are marked with high levels of acetylated histones. In addition, our studies suggest that DNA methylation and nucleosome occupancy patterns restrict access of Kaiso to potential binding sites and influence cell type-specific binding.

Conclusions

We propose a new model for the genome-wide binding and function of Kaiso whereby Kaiso binds to unmethylated regulatory regions and contributes to the active state of target promoters.

【 授权许可】

   
2013 Blattler et al.; licensee BioMed Central Ltd.

【 图 表 】

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