Neurons of self-defence: neuronal innervation of the exocrine defence glands in stick insects

Frontiers in Zoology

Johannes Strauß¹  Tina E. Trenczek²  Reinhard Lakes-Harlan¹  Yvette M. von Bredow²  Christoph-Rüdiger von Bredow²  Konrad Stolz² 

[1] AG Integrative Sensory Physiology, Institute for Animal Physiology, Justus-Liebig-Universität Gießen, Gießen, Germany;Institute for General and Applied Zoology, Justus-Liebig-Universität Gießen, Gießen, Germany

关键词: Defence behaviour;    Motoneuron;    Neuronal tracing;    Stick insect;    Defence glands;    Neuronal innervation;    Neuroanatomy;    Insect;   

Others  :  1232728
DOI  :  10.1186/s12983-015-0122-0

 received in 2015-04-26, accepted in 2015-10-08,  发布年份 2015

【 摘 要 】

Background

Stick insects (Phasmatodea) use repellent chemical substances (allomones) for defence which are released from so-called defence glands in the prothorax. These glands differ in size between species, and are under neuronal control from the CNS. The detailed neural innervation and possible differences between species are not studied so far. Using axonal tracing, the neuronal innervation is investigated comparing four species. The aim is to document the complexity of defence gland innervation in peripheral nerves and central motoneurons in stick insects.

Results

In the species studied here, the defence gland is innervated by the intersegmental nerve complex (ISN) which is formed by three nerves from the prothoracic (T1) and suboesophageal ganglion (SOG), as well as a distinct suboesophageal nerve (Nervus anterior of the suboesophageal ganglion). In Carausius morosus and Sipyloidea sipylus, axonal tracing confirmed an innervation of the defence glands by this N. anterior SOG as well as N. anterior T1 and N. posterior SOG from the intersegmental nerve complex. In Peruphasma schultei, which has rather large defence glands, only the innervation by the N. anterior SOG was documented by axonal tracing. In the central nervous system of all species, 3-4 neuron types are identified by axonal tracing which send axons in the N. anterior SOG likely innervating the defence gland as well as adjacent muscles. These neurons are mainly suboesophageal neurons with one intersegmental neuron located in the prothoracic ganglion. The neuron types are conserved in the species studied, but the combination of neuron types is not identical. In addition, the central nervous system in S. sipylus contains one suboesophageal and one prothoracic neuron type with axons in the intersegmental nerve complex contacting the defence gland.

Conclusions

Axonal tracing shows a very complex innervation pattern of the defence glands of Phasmatodea which contains different neurons in different nerves from two adjacent body segments. The gland size correlates to the size of a neuron soma in the suboesophageal ganglion, which likely controls gland contraction. In P. schultei, the innervation pattern appears simplified to the anterior suboesophageal nerve. Hence, some evolutionary changes are notable in a conserved neuronal network.

【 授权许可】

   
2015 Stolz et al.

【 图 表 】

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