Prevalence of African swine fever virus in apparently healthy domestic pigs in Uganda

BMC Veterinary Research

Lonzy Ojok²  William Olaho-Mukani³  Julius Boniface Okuni²  Frank Norbert Mwiine²  Savannah Mwesigwa²  Sylvester Ochwo²  Mathias Afayoa²  David Kalenzi Atuhaire¹ 

[1] National Agricultural Research Organization, National Livestock Resources Research Institute, P.O.BOX 96, Tororo, Uganda;College of Veterinary Medicine, Animal resources and Biosecurity, Makerere University, P.O.BOX 7062, Kampala, Uganda;African Union-Interafrican Bureau for Animal Resources, P.O.BOX 30786, Nairobi, Kenya

关键词: Surveillance;    Slaughter pigs;    Seropositive;    Prevalence;    PCR;    ELISA;    African swine fever virus;   

Others  :  1119375
DOI  :  10.1186/1746-6148-9-263

 received in 2013-07-17, accepted in 2013-12-23,  发布年份 2013

【 摘 要 】

Background

African swine fever (ASF) is a contagious viral disease which can cause up to 100% mortality among domestic pigs leading to serious socio-economic impact on people’s livelihoods. ASF is endemic in Uganda and there is paucity of information on the epidemiology of the disease. The major aim of this study was to determine the seroprevalence and prevalence of African swine fever virus (ASFV) in apparently healthy slaughter pigs at Wambizi slaughterhouse in Kampala city, Uganda. We also estimated the presence of ASFV antibodies and circulating viral antigens in pigs from selected districts of Uganda during targeted surveillance. We analysed 540 and 181 blood samples collected from slaughter pigs and pigs from targeted surveillance districts respectively.

Results

The prevalence of ASFV in slaughter pigs was 52.96% (95% CI, 48.75-57.14) and 11.5% (95% CI, 9.06-14.45) by ELISA and PCR respectively. In surveillance districts, the proportion of ASFV positive pigs was 53.59% (95% CI, 46.33-60.71) and 0.55% (95% CI, 0.1-3.06) by ELISA and PCR respectively.

Conclusion

The study has found out a high seroprevalence of ASFV antibodies in apparently healthy slaughter pigs and also a high proportion of ASFV antibody seropositive pigs in surveyed districts in Uganda indicating exposure to ASFV. However, there was a lower prevalence of ASFV infection implying that there could be low virulent strains of ASFV circulating in domestic pigs in Uganda which requires further investigation.

【 授权许可】

   
2013 Atuhaire et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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