Accumulation of intermediate denitrifying compounds inhibiting biological denitrification on cathode in Microbial Fuel Cell

Journal of Environmental Health Science Engineering

Mahad Said Baawain¹  Abdullah Al-Mamun² 

[1]Centre for Environmental Studies and Research, Sultan Qaboos University, Al-khodh, P.C. 123, Muscat, Muscat, Sultanate of Oman

[2]Department of Civil & Architectural engineering, Sultan Qaboos University, Al-Khodh, P.C. 123, Muscat, Sultanate of Oman

关键词: Process inhibition;    Bioremediation;    Biological denitrification;    Bio-cathode;    Microbial fuel cell;   

Others  :  1234470
DOI  :  10.1186/s40201-015-0236-5

 received in 2015-05-04, accepted in 2015-11-12,  发布年份 2015

【 摘 要 】

Background

Bio-cathode denitrifying microbial fuel cell (MFC) is a promising bio-electrochemical system (BES) where both the reactions of anodic oxidation and cathodic reduction are catalyzed by microorganisms. In this nitrogen removal process, a complete biological denitrification from nitrate (NO ₃^- ) to molecular nitrogen (N ₂ ) was achieved by four reduction steps, forming nitrite (NO ₂⁻ ), nitric oxide (NO) and nitrous oxide (N ₂ O) as intermediate compounds. These enzymatic catalysis reductions are often slowed down on cathode electrode at the higher cathodic nitrate loading. This study investigated the cause for inhibition of the biological denitrification in a three-chambered MFC where the middle chamber acted as denitrifying bio-cathode and the two chambers at the side acted as bio-anode. Carbon fiber brushes were used as electrodes and nafion membranes were used as separator between the chambers.

Results

The maximum power obtained was 14.63 W m ⁻³net cathodic compartment (NCC) (R _ext=11.5Ω) at an optimum nitrate loading of 0.15 kg NO ₃⁻ -N m ⁻³NCC d ⁻¹ . The accumulation of one of the intermediate denitrifying compound, e.g., NO ₂⁻adversely affected biological denitrification rate on cathode. According to chemical kinetics, the accumulated NO ₂⁻will form free nitrous acid (FNA, HNO ₂ ) in aqueous chemical system spontaneously. The study showed that approximately 45 % of the current production and 20 % of the total denitrification was decreased at a FNA concentration of 0.0014 ± 0.0001 mg HNO ₂– N L ⁻¹with an equivalent nitrite concentration of 6.2 ± 0.9 mg NO ₂^-- N L ⁻¹ .

Conclusions

The novel biological process indicates the potential of using denitrifying bio-cathode MFC for green energy production.

【 授权许可】

   
2015 Al-Mamun and Baawain.

【 图 表 】

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