Differentiated embryonic chondrocyte-expressed gene 1 is associated with hypoxia-inducible factor 1α and Ki67 in human gastric cancer

Diagnostic Pathology

Yun-Shan Wang¹  Ruan-Li Hong²  Shu-Yi Han⁴  Yan Zheng⁴  Yi Kong³  Rui Hu³  Yan-Yan Song³  Xiao-Li Ma⁴  Dong-Jie Xiao⁴  Yan-Fei Jia¹ 

[1] Shandong Province Key Lab of Tumor Target Molecule, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013,, Shandong Province, China;Gynecology and obstetrics, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013,, Shandong Province, China;Shandong University School of Medicine, Jinan, 250012,, Shandong Province, China;Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan, 250013,, Shandong Province, China

关键词: Immunohistochemistry;    Gastric cancer;    Proliferation;    Hypoxia-inducible factor 1α;    Differentiated embryo chondrocyte 1;   

Others  :  807426
DOI  :  10.1186/1746-1596-8-37

 received in 2013-01-09, accepted in 2013-02-16,  发布年份 2013

【 摘 要 】

Background

Gastric cancer is a leading causes of cancer-related deaths ,but the underlying molecular mechanisms of its progression are largely unknown. Differentiated embryonic chondrocyte-expressed gene 1 (DEC1), is an important transcription factor involved in the progression of tumors and has recently been identified to be strongly inducible by hypoxia. Little is known about the contribution of DEC1 to the intracellular hypoxia and proliferation signaling events in gastric cancer.

Methods

Immunohistochemistry was used to detect the expression of DEC1, hypoxia-inducible factor 1(HIF-1α) and Ki67 in 173 human gastric cancer samples and adjacent non-tumor tissues samples. The relationship between DEC1, HIF-1α and Ki67 was evaluated.

Results

DEC1 protein was persistently expressed in the nucleus and cytoplasm of gastric cancer tissue. The protein expression of DEC1 and HIF-1α in tumour tissues was 83.8% and 54.3%, respectively, and was significantly higher than that in adjacent normal tissues (83.8% vs 23.7%, P <0.001; 54.3% vs 12.7%, P< 0.001). The expression of DEC1 and HIF-1α was associated with poor histological differentiation. (P < 0. 01). Furthermore, DEC1 level was positively correlated with HIF-1α (P < 0. 01, r=0.290) and Ki67 expression (P < 0. 01, r=0.249).

Conclusion

The upregulation of DEC1 may play an important role in hypoxia regulation and cell proliferation in gastric cancer. The relevant molecular mechanism requires further investigation.

Virtual Slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1794565980889391 webcite

【 授权许可】

   
2013 Jia et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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