A serological investigation of caseous lymphadenitis in four flocks of sheep

Irish Veterinary Journal

Fiona EA Murdock²  Kath M O'Reilly⁵  Graham J Baird³  David C King⁴  Elbarte M Kamp¹  Seán A Fee⁴  Frank E Malone⁴ 

[1] Institute of Animal Science and Health (ID-Lelystad), PO Box 65, 8200 AB Lelystad, The Netherlands;Department of Agriculture and Rural Development for Northern Ireland, Veterinary Service, Dundonald House, Upper Newtownards Road, Belfast, Northern Ireland BT4 3SB;Scottish Agricultural College, Veterinary Science Division, Greycrook, St. Boswells, Melrose, Scotland, TD6 0EQ;Department of Agriculture and Rural Development for Northern Ireland, Veterinary Sciences Division, 43 Beltany Road, Omagh, County Tyrone, Northern Ireland, BT78 5NF;Ecology and Epidemiology Group, Biological Sciences, University of Warwick, Coventry, England, CV4 7AL

关键词: Corynebacterium pseudotuberculosis;    Serology;    Caseous lymphadenitis;    Sheep;   

Others  :  1150547
DOI  :  10.1186/2046-0481-59-1-19

【 摘 要 】

A double antibody sandwich ELISA developed by ID-DLO, Lelystad to detect Corynebacterium pseudotuberculosis infection was used on 329 sheep from four pedigree Suffolk flocks in which clinical cases of caseous lymphadenitis (CLA) had occurred. At subsequent necropsy, typical CLA lesions were seen in 133 sheep, and the diagnosis was confirmed on culture. Lesions were most commonly seen in lungs (n = 46), parotid lymph nodes (n = 44), prescapular lymph nodes (n = 38) and mediastinal lymph nodes (n = 31). The sensitivity of the ELISA test for detecting culture-positive sheep was 0.88, while the specificity of the test was 0.55. The antibody ELISA detected 87.5 per cent of sheep that had CLA lesions restricted to internal organs only. It was concluded that the ELISA test has a valuable role in detecting sheep with both clinical and subclinical CLA.

   

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Baird GJ: Current perspectives on caseous lymphadenitis. Practice 2003, 25:62-68.
• [2]Batey RG: Pathogenesis of caseous lymphadenitis in sheep and goats. Australian Veterinary Journal 1986, 63:269-272.
• [3]Brown CC, Olander HJ: Caseous lymphadenitis of goats and sheep: a review. Veterinary Bulletin 1987, 57:1-12.
• [4]Cowan ST, Steel KJ: Cowan and Steel's Manual for the Identification of Medical Bacteria. Second edition. Cambridge: Cambridge University Press; 1974.
• [5]Dercksen DP, Brinkhof JMA, Dekker-Norren T, van Maanen K, Bode CF, Baird G, Kamp EM: A comparison of four serological tests for the diagnosis of caseous lymphadenitis in sheep and goats. Veterinary Microbiology 2000, 75:167-175.