Bioremediation of p-Nitrophenol by Pseudomonas putida 1274 strain

Journal of Environmental Health Science Engineering

Alka Mehta²  Akella Sivaramakrishna¹  Melvin S Samuel² 

[1] School of Advance Sciences, VIT University, Vellore 632014, Tamil Nadu, India;School of Biosciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India

关键词: Bioremediation;    Biodegradation;    Hydroquinone;    Pseudomonas putida;    p-Nitrophenol;   

Others  :  805395
DOI  :  10.1186/2052-336X-12-53

 received in 2013-03-14, accepted in 2014-02-19,  发布年份 2014

【 摘 要 】

Background

p-Nitrophenol (PNP) occurs as contaminants of industrial effluents and it is the most important environmental pollutant and causes significant health and environmental risks, because it is toxic to many living organisms. Nevertheless, the information regarding PNP degradation pathways and their enzymes remain limited.

Objective

To evaluate the efficacy of the Pseudomonas Putida 1274 for removal of PNP.

Methods

P. putida MTCC 1274 was obtained from MTCC Chandigarh, India and cultured in the minimal medium in the presence of PNP. PNP degradation efficiency was compared under different pH and temperature ranges. The degraded product was isolated and analyzed with different chromatographic and spectroscopic techniques.

Results

P. putida 1274 shows good growth and PNP degradation at 37°C in neutral pH. Acidic and alkali pH retarded the growth of P. putida as well as the PNP degradation. On the basis of specialized techniques, hydroquinone was identified as major degraded product. The pathway was identified for the biodegradation of PNP. It involved initial removal of the nitrate group and formation of hydroquinone as one of the intermediates.

Conclusion

Our results suggested that P. putida 1274 strain would be a suitable aspirant for bioremediation of nitro-aromatic compounds contaminated sites in the environment.

【 授权许可】

   
2014 Samuel et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

Figure 5.

Figure 6.

Figure 7.

【 参考文献 】

• [1]Spain JC, Gibson DT: Pathway for biodegradation of para-nitrophenol in a Moraxella sp. Appl Environ Microbiol 1991, 57:812-819.
• [2]Mulbry WW, Karns JS: Purification and characterization of three parathion hydrolases from gram-negative bacterial strains. Appl Environ Microbiol 1989, 55:289-293.
• [3]Schackmann A, Muller R: Reduction of nitroaromatic compounds by different Pseudomonos species under aerobic conditions. Appl Microbiol Biotechnol 1991, 34:809-813.
• [4]Guliy OI, Ignatov OV, Makarov OE, Ignatovet VV: Determination of organophosphorus aromatic nitro insecticides and p-nitrophenol by microbial-cell respiratory activity. Biosens Bioelectron 2002, 8:1005-1013.
• [5]Kitagawa W, Kimura N, Kamagata Y: A novel p-nitrophenol degradation gene cluster from a gram-positive bacterium Rhodococcus opacus SAO101. J Bacteriol 2004, 186:4894-4902.
• [6]Leung KT, Tresse O, Errampalli D, Lee H, Trevors JT: Mineralization of p-nitrophenol by pentachloro-phenol-degrading Sphingomonas spp. FEMS Microbiol Lett 1997, 155:107-114.
• [7]US Environmental Protection Agency: Quality Criteria for Water. Washington, DC: US Environmental Protection Agency; 1986.
• [8]Zylstra GJ, Bang SW, Newman LM, Perry LL: Biodegradation of Nitroaromatic Compounds and Explosives. Boca Raton, Florida: Lewis Publishers; 2000:145-160. [Microbial Degradation of Mononitrophenols and Mononitrobenzoates]
• [9]Takahashi N, Nakai T, Satoh Y, Katoh Y: Variation of biodegradability of nitrogenous organic compounds by ozonation. Wat Res 1994, 28:1563-1570.
• [10]Kalbende PP, Tarase MV, Zade AB: Preparation, characterization, and thermal degradation studies of p-Nitrophenol-based copolymer. J Chem 2013, 1:9.
• [11]Peng S, Zhao L, Liu X, Chen D: The oxidative degradation by pyrolusite of p-nitrophenol wastewater after micro-electrolysis pretreatment. Int J Eng Sci Tech 2010, 2:104-109.
• [12]Kiwi J, Pulgarin C, Peringer P: Effect of Fenton and photo- Fenton reaction on the degradation and biodegradability of 2- and 4-nitrophenols in water treatment. Appl Catal B Environ 1994, 3:335-350.
• [13]Bhushan B, Chauhan A, Samanta SK, Jain RK: Kinetics of biodegradation of p-nitrophenol by different bacteria. Biochem Biophys Res Commun 2000, 274:626-630.
• [14]Timmis KN, Steffan RJ, Unterman R: Designing microorganisms for the treatment of toxic wastes. Annu Rev Microbiol 1994, 48:525-557.
• [15]Kadiyala V, Spain JC: A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and the oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905. Appl Environ Microbiol 1998, 64:2479-2484.
• [16]Zaidi BR, Mehta NK: Inoculations of indigenous and nonindigenous bacteria to enhance biodegradation of p-nitrophenol in industrial wastewater: effect of glucose as second substrate. Biotechnol Lett 1996, 18:565-570.
• [17]Zhang S, Sun W, Xu L, Zheng X, Chu X, Tian J, Wu N, Fan Y: Identification of the para-nitrophenol catabolic pathway, and characterization of three enzymes involved in the hydroquinone pathway, in pseudomonas sp. 1–7. BMC Microbiol 2012, 12:27. BioMed Central Full Text
• [18]Liu Z, Yang C, Qiao C: Biodegradation of p -nitrophenol and 4-chlorophenol by Stenotrophomonas sp. FEMS Microbiol Lett 2007, 277:150-156.
• [19]Jain RK, Dreisbach JH, Spain JC: Biodegradation of 4- nitrophenol via 1,2 4, -benzenetrial by an Arthrobacter sp. Appl Environ Microbiol 1994, 60:3030-3032.
• [20]Arora PK: Metabolism of para-nitrophenol in Arthrobacter sp. J Environ Res Manag 2012, 3(3):052-057.
• [21]Chauhan A, Chakraborti AK, Jain RK: Plasmid encoded degradation of p-nitrophenol and 4-nitrocatechol by Arthrobacter protophormiae. Biochem Biophys Res Commun 2000, 270(3):733-740.
• [22]Meenal K, Ambalal C: Efficient P. putida for degradation of p-nitrophenol. Indian J Biotechnol 2006, 5:411-415.
• [23]Wei M, Zhang J-J, Liu H, Zhou N-Y: para-Nitrophenol 4-monooxygenase and hydroxyquinol 1,2-dioxygenase catalyze sequential transformation of 4-nitrocatechol in Pseudomonas sp. strain WBC-3. Biodegradation 2010, 21:915-921.
• [24]Zheng Y, Xua S, Yuana Y, Lia W, Zhong Y, Xonga L, Liu D: Biodegradation of p-nitrophenol by Pseudomonas aeruginosa HS-D38. IEEE 2008, 978:4497-4500.
• [25]Zhang JJ, Liu H, Xiao Y, Zhang XE, Zhou NY: Identification and characterization of catabolic para-nitrophenol4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC-3. J Bacteriol 2009, 191(8):2703-2710.
• [26]Pirie P, Naeimpoor F, Hejazi P: A microcosm study on P-Nitrophenol biodegradation in soil slurry by Alcaligenes faecalis: Plackett-Burman Design. Iran J Chem Eng 2011, 8:57-68.
• [27]Leilei Z, Mingxin H, Suiyi Z: Biodegradation of p-nitrophenol by Immobilized Rhodococcus sp. Strain Y-1. Chem Biochem Eng 2012, 26:137-144.
• [28]Samuel MS, Sivaramakrishna A, Mehta A: Degradation and detoxification of aflatoxin B1 by Pseudomonas putida. IBB 2013, 86:202-209.
• [29]Samuel MS, Aiko V, Panda P, Mehta A: Aflatoxin B1 occurance, Biosynthesis and its Degradation. JPAM 2013, 7:1-7.
• [30]Kulkarni M, Chaudhari A: Microbial remediation of nitro-aromatic compounds: an overview. J Environ Manage 2007, 85:496-512.
• [31]Bruhn C, Lenke H, Knackmuss HJ: Nitrosubstituted aromatic compounds as nitrogen source for bacteria. Appl Environ Microbiol 1987, 53:208-210.
• [32]Labana S, Singh OV, Basu A, Pandey G, Jain RK: A microcosm study on bioremediation of p-nitrophenolcontaminated soil using Arthrobacter protophormiae RKJ100. Appl Microbiol Biotechnol 2005, 68:417-424.
• [33]Borras C, Laredo T, Mostany J, Scharifker BR: Study of the oxidation of solutions of p-chlorophenol and p-nitrophenol on Bi-doped PbO2 electrodes by UV–vis and FTIR in situ spectroscopy. Electrochim Acta 2004, 49:641-648.
• [34]Holdway DA, Dixon DG, Kaiser KL: The acute toxicity of pulse-dosed, para-substituted phenols to larval American flag fish (Jordanella floridae): a comparison with toxicity to photoluminescent bacteria and predicted toxicity using log Kow. Sci Total Environ 1991, 104(3):229-237.
• [35]Qiu X, Zhong Q, Li M, Bai W, Li B: Biodegradation of p-nitrophenol by methyl parathion-degrading Ochrobactrum sp. B2. Int Biodeterioration Biodegrad 2007, 59:297-301.