Effects of indole on drug resistance and virulence of Salmonella enterica serovar Typhimurium revealed by genome-wide analyses

Gut Pathogens

Kunihiko Nishino³  Axel Cloeckaert¹  Akihito Yamaguchi²  Tatsuya Takagi²  Kousuke Okamoto²  Agnès Wiedemann¹  Suguru Yamasaki²  Sylvie Baucheron¹  Etienne Giraud¹  Eiji Nikaido² 

[1] Université François Rabelais de Tours, UMR1282 Infectiologie et Santé Publique, F-37000, Tours, France;Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan;Laboratory of Microbiology and Infectious Diseases, Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka, 567-00447, Japan

关键词: SPI-1;    Salmonella;    RamA;    Indole;    AcrAB;   

Others  :  822039
DOI  :  10.1186/1757-4749-4-5

 received in 2012-04-24, accepted in 2012-05-25,  发布年份 2012

【 摘 要 】

Background

Many Gram-positive and Gram-negative bacteria produce large quantities of indole as an intercellular signal in microbial communities. Indole demonstrated to affect gene expression in Escherichia coli as an intra-species signaling molecule. In contrast to E. coli, Salmonella does not produce indole because it does not harbor tnaA, which encodes the enzyme responsible for tryptophan metabolism. Our previous study demonstrated that E. coli-conditioned medium and indole induce expression of the AcrAB multidrug efflux pump in Salmonella enterica serovar Typhimurium for inter-species communication; however, the global effect of indole on genes in Salmonella remains unknown.

Results

To understand the complete picture of genes regulated by indole, we performed DNA microarray analysis of genes in the S. enterica serovar Typhimurium strain ATCC 14028s affected by indole. Predicted Salmonella phenotypes affected by indole based on the microarray data were also examined in this study. Indole induced expression of genes related to efflux-mediated multidrug resistance, including ramA and acrAB, and repressed those related to host cell invasion encoded in the Salmonella pathogenicity island 1, and flagella production. Reduction of invasive activity and motility of Salmonella by indole was also observed phenotypically.

Conclusion

Our results suggest that indole is an important signaling molecule for inter-species communication to control drug resistance and virulence of S. enterica.

【 授权许可】

   
2012 Nikaido et al.; licensee BioMed Central Ltd.

【 图 表 】

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