QSAR study and the hydrolysis activity prediction of three alkaline lipases from different lipase-producing microorganisms

Lipids in Health and Disease

Heng Zheng²  Changlu Guo¹  Yujie Dai³  Yehong Zhang³  Xiaolu Li³  Xiaojie Wang³  Haikuan Wang³ 

[1] College of Oceanography, Harbin Institute of Technology, Weihai, 264209, P. R. China;School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, P. R. China;Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, P. R. China

关键词: Vegetable oils;    Lipase activity;    Ester;    GFA;    QSAR;   

Others  :  1160194
DOI  :  10.1186/1476-511X-11-124

 received in 2012-08-14, accepted in 2012-09-12,  发布年份 2012

【 摘 要 】

The hydrolysis activities of three alkaline lipases, L-A1, L-A2 and L-A3 secreted by different lipase-producing microorganisms isolated from the Bay of Bohai, P. R. China were characterized with 16 kinds of esters. It was found that all the lipases have the ability to catalyze the hydrolysis of the glycerides, methyl esters, ethyl esters, especially for triglycerides, which shows that they have broad substrate spectra, and this property is very important for them to be used in detergent industry. Three QSAR models were built for L-A1, L-A2 and L-A3 respectively with GFA using Discovery studio 2.1. The models equations 1, 2 and 3 can explain 95.80%, 97.45% and 97.09% of the variances (R²_adj) respectively while they could predict 95.44%, 89.61% and 93.41% of the variances (R²_cv) respectively. With these models the hydrolysis activities of these lipases to mixed esters were predicted and the result showed that the predicted values are in good agreement with the measured values, which indicates that this method can be used as a simple tool to predict the lipase activities for single or mixed esters.

【 授权许可】

   
2012 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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