Hyaluronan production and chondrogenic properties of primary human chondrocyte on gelatin based hematostatic spongostan scaffold

Journal of Orthopaedic Surgery and Research

Peraphan Pothacharoen²  Prachya Kongtawelert²  Sattaya Rojanasthien³  Olarn Arpornchayanon³  Taninnit Leerapun³  Dumnoensun Pruksakorn³  Jongkolnee Settakorn¹  Puwapong Nimkingratana³  Jeerawan Klangjorhor² 

[1] Department of Pathology, Faculty of Medicine, Chiang Mai University, Intravarorot Road, Sripoom, Chiang Mai, 50200, Thailand;Thailand Excellence Center for Tissue Engineering and Stem Cells, Faculty of Medicine, Chiang Mai University, Intravarorot Road, Sripoom, Chiang Mai, 50200, Thailand;Musculoskeletal Research Laboratory, Department of Orthopedics, Faculty of Medicine, Chiang Mai University, Intravarorot Road, Sripoom, Chiang Mai, 50200, Thailand

关键词: Scaffold;    Spongostan;    Gelatin;    Collagen;    Hyaluronan;    Human articular chondrocyte 3D culture;   

Others  :  817819
DOI  :  10.1186/1749-799X-7-40

 received in 2011-10-01, accepted in 2012-12-05,  发布年份 2012

【 摘 要 】

Background

Autologous chondrocyte transplantation is a promising technique for treatment of cartilage defects. Three dimensional chondrocyte cultures on a scaffold are widely used to retain the chondrogenic phenotype. Using a biodegradable gelatin scaffold is one option for the cell delivery system, but molecular and histological studies of the method have not yet been done.

Methods

We evaluated the chondrogenic property of the primary human chondrocyte on a gelatin scaffold as compared to a collagen scaffold over a period of 21 days. We examined the production of glycosaminoglycan by quantitative and histological analysis. Gene expression of cartilage-associated molecules was assessed by quantitative RT-PCR.

Results

The gelatin scaffold showed the ability to promote chondrocyte expansion, chondrogenic phenotype retention at molecular and mRNA levels.

Conclusions

This scaffold is thus suitable for use as an in vitro model for chondrocyte 3D culture.

【 授权许可】

   
2012 Klangjorhor et al.; licensee BioMed Central Ltd.

【 图 表 】

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