Postprandial enrichment of triacylglycerol-rich lipoproteins with omega-3 fatty acids: lack of an interaction with apolipoprotein E genotype?

Lipids in Health and Disease

Mélanie Plourde¹  Julie A Lovegrove³  Kim G Jackson³  Anne-Marie Minihane²  Marie-Julie Allard¹  Valérie Conway¹ 

[1] Centre de recherche sur le vieillissement, Institut Universitaire de Gériatrie de Sherbrooke, Université de Sherbrooke, 1036 Belvédère Sud, Sherbrooke J1H 4C4, Canada;Nutrition Department, Norwich Medical School, University of East Anglia, Norwich, UK;Hugh Sinclair Unit of Human Nutrition and Institute for Cardiovascular and Metabolic Research (ICMR), University of Reading, Reading, UK

关键词: Postprandial lipemia;    Fish-oil supplementation;    VLDL;    n − 3 PUFA metabolism;    Chylomicrons;    APOE4 carriers;   

Others  :  1152048
DOI  :  10.1186/1476-511X-13-148

 received in 2014-04-23, accepted in 2014-09-09,  发布年份 2014

【 摘 要 】

Background

We have previously demonstrated that carrying the apolipoprotein (apo) E epsilon 4 (E4+) genotype disrupts omega-3 fatty acids (n − 3 PUFA) metabolism. Here we hypothesise that the postprandial clearance of n − 3 PUFA from the circulation is faster in E4+ compared to non-carriers (E4−). The objective of the study was to investigate the fasted and postprandial fatty acid (FA) profile of triacylglycerol-rich lipoprotein (TRL) fractions: S_f >400 (predominately chylomicron CM), S_f 60 − 400 (VLDL₁), and S_f 20 − 60 (VLDL₂) according to APOE genotype.

Methods

Postprandial TRL fractions were obtained in 11 E4+ (ϵ3/ϵ4) and 12 E4− (ϵ3/ϵ3) male from the SATgenϵ study following high saturated fat diet + 3.45 g/d of docosahexaenoic acid (DHA) for 8-wk. Blood samples were taken at fasting and 5-h after consuming a test-meal representative of the dietary intervention. FA were characterized by gas chromatography.

Results

At fasting, there was a 2-fold higher ratio of eicosapentaenoic acid (EPA) to arachidonic acid (P = 0.046) as well as a trend towards higher relative% of EPA (P = 0.063) in the S_f >400 fraction of E4+. Total n − 3 PUFA in the S_f 60 − 400 and S_f 20 − 60 fractions were not APOE genotype dependant. At 5 h, there was a trend towards a time × genotype interaction (P = 0.081) for EPA in the S_f >400 fraction. When sub-groups were form based on the level of EPA at baseline within the S_f >400 fraction, postprandial EPA (%) was significantly reduced only in the high-EPA group. EPA at baseline significantly predicted the postprandial response in EPA only in E4+ subjects (R² = 0.816).

Conclusion

Despite the DHA supplement contain very low levels of EPA, E4+ subjects with high EPA at fasting potentially have disrupted postprandial n − 3 PUFA metabolism after receiving a high-dose of DHA.

Trial registration

Registered at clinicaltrials.gov/show/NCT01544855.

【 授权许可】

   
2014 Conway et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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