Escherichia coli challenge and one type of smectite alter intestinal barrier of pigs

Journal of Animal Science and Biotechnology

James Eugene Pettigrew³  Orlando Osuna²  Carol Wolfgang Maddox⁴  H Rex Gaskins³  Jeong Jae Lee³  Minho Song¹  Yanhong Liu³  Juliana Abranches Soares Almeida³ 

[1] Current address: Department of Animal Science and Biotechnology, Chungnam National University, Daejeon, South Korea;Milwhite, Inc., Brownsville, TX, USA;Department of Animal Sciences, University of Illinois, Urbana 61801, USA;Department of Pathobiology, University of Illinois, Urbana 61801, USA

关键词: Zeolite;    Smectite;    Pigs;    E. coli;    Barrier function;   

Others  :  804833
DOI  :  10.1186/2049-1891-4-52

 received in 2013-07-24, accepted in 2013-12-05,  发布年份 2013

【 摘 要 】

An experiment was conducted to determine how an E. coli challenge and dietary clays affect the intestinal barrier of pigs. Two groups of 32 pigs (initial BW: 6.9 ± 1.0 kg) were distributed in a 2 × 4 factorial arrangement of a randomized complete block design (2 challenge treatments: sham or E. coli, and 4 dietary treatments: control, 0.3% smectite A, 0.3% smectite B and 0.3% zeolite), with 8 replicates total. Diarrhea score, growth performance, goblet cell size and number, bacterial translocation from intestinal lumen to lymph nodes, intestinal morphology, and relative amounts of sulfo and sialo mucins were measured. The E. coli challenge reduced performance, increased goblet cell size and number in the ileum, increased bacterial translocation from the intestinal lumen to the lymph nodes, and increased ileal crypt depth. One of the clays (smectite A) tended to increase goblet cell size in ileum, which may indicate enhanced protection. In conclusion, E. coli infection degrades intestinal barrier integrity but smectite A may enhance it.

【 授权许可】

   
2013 Almeida et al.; licensee BioMed Central Ltd.

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